目的:建立复方烫伤软膏的质量标准。方法:运用TLC对制剂中的大黄、黄柏进行定性鉴别;运用HPLC测定制剂中黄芩苷的含量。结果:薄层斑点清晰、重现性好,可以检测出大黄、黄柏的特征斑点;含量测定准确、稳定,黄芩苷进样量在3.02~24.16μg内呈良好的线性关系,黄芩苷的平均回收率为97.47%,RSD为1.7%。结论:所建方法专属性强,定量方法简便、准确,可以作为制订复方烫伤软膏的质量标准的依据。 Objective: To establish the quality standard of compound scald ointment. Methods: TLC was used to identify the rhubarb and cortex phellodendri in the preparation. The content of baicalin in the preparation was determined by HPLC. Results: The TLC spots were clear and the reproducibility was good. The spots of Rhubarb and Phellodendron were detected. The content of baicalin was accurate and stable. The linearity of baicalin injection was in the range of 3.02-24.16 μg. The average recovery of baicalin The rate was 97.47% and the RSD was 1.7%. Conclusion: The proposed method is specific and specific, and the method is simple and accurate. It can be used as the basis for formulating the quality standard of compound scald ointment.