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目的:观察大黄对急性百草枯中毒大鼠TNF-α、IL-10的干预作用,探讨其可能的作用机制。方法:90只SD大鼠随机分为生理盐水对照组(A组)、PQ(60 mg/kg)灌胃染毒组(B组)、生大黄(300mg/kg.d)干预组(C组),每组30只。中毒后6h、24h、72h分批处死存活的大鼠,并且检测大鼠血浆TNF-α、IL-10水平。结果:B组、C组TNF-α、IL-10水平在染毒后6h开始升高,72h达到高峰,与A组相比,差异有统计学意义(P<0.05、P<0.01),在相同时间点C组TNF-α和IL-10的表达低于B组,差异均有统计学意义(P<0.01)。B组、C组血浆TNF-α、IL-10水平与中毒时间呈显著正性相关关系(r=0.849,P<0.01;r=0.790,P<0.01;r=0.0.943,P<0.01;r=0.892,P<0.01)。结论:大黄能够通过降低百草枯中毒大鼠体内的TNF-α、IL-10水平,减轻百草枯对大鼠的损伤作用。
Objective: To observe the effect of rhubarb on TNF-α and IL-10 in acute paraquat poisoning rats and to explore its possible mechanism. Methods: Ninety Sprague-Dawley rats were randomly divided into saline control group (A group), PQ (60 mg / kg) intragastric administration group (B), rhubarb (300mg / kg.d) intervention group ), 30 in each group. After poisoning 6h, 24h, 72h, rats were sacrificed in batches in batches, and the level of plasma TNF-α and IL-10 in rats was measured. Results: The levels of TNF-α and IL-10 in group B and group C increased at 6h and peaked at 72h, which were significantly different from those in group A (P <0.05, P <0.01) At the same time point, the expression of TNF-α and IL-10 in group C was lower than that in group B, the differences were statistically significant (P <0.01). The plasma levels of TNF-α and IL-10 in group B and group C were positively correlated with poisoning time (r = 0.849, P <0.01; r = 0.790, r = 0.892, P <0.01). Conclusion: Rhubarb can reduce paraquat poisoning in rats by the level of TNF-α, IL-10 levels, reduce paraquat injury in rats.