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目的探讨刺糖粗多糖对糖尿病大鼠降糖及抗氧化功能。方法采用水提醇沉法提取刺糖粗多糖。采用健康雄性昆明小鼠90只,随机取出8只作为空白对照组,注射蒸馏水0.2ml/10g,剩余小鼠制备糖尿病小鼠模型。将糖尿病小鼠随机分为5组:模型组、阳性药物组(盐酸二甲双胍,300mg/kg)、刺糖粗多糖低剂量组(200mg/kg)、中剂量组(400mg/kg)、高剂量组(800mg/kg)。于给药前、给药第2、4周测定小鼠血糖,测定并比较小鼠血清及肝脏中丙二醛(MDA)和总超氧化物歧化酶(T-SOD)的含量测定。结果刺糖粗多糖的最大给药量为9.596g/kg。给药前和给药后第2周、第4周模型组、阳性药物组、刺糖粗多糖低、中、高剂量组小鼠体质量低于空白对照组,差异有统计学意义(P<0.05)。给药后第2周及第4周阳性药物组小鼠空腹血糖低于模型组,差异有统计学意义(P<0.05)。给药后第4周刺糖粗多糖高剂量组小鼠空腹血糖高于模型组、糖粗多糖低、中剂量组,差异有统计学意义(P<0.05)。各组小鼠血清及肝脏中T-SOD含量差异无统计学意义(P>0.05)。模型组小鼠血清及肝脏中MDA高于空白对照组,差异均有统计学意义(P<0.05)。阳性药物组及刺糖粗多糖低、中、高剂量组小鼠血清及肝脏中MDA含量低于模型组,差异有统计学意义(P<0.05)。刺糖粗多糖高剂量组小鼠肝脏中MDA低于刺糖粗多糖中、低剂量组,差异有统计学意义(P<0.05)。结论刺糖粗多糖具有潜在降血糖作用,其降血糖机制可能与氧化应激能力有关。
Objective To investigate the hypoglycemic and anti-oxidative effects of polysaccharides from Diabetic rats on diabetic rats. Methods Water extraction and alcohol precipitation extraction of polysaccharides. Ninety healthy male Kunming mice were randomly selected. Eight rabbits were randomly divided into blank control group and distilled water 0.2ml / 10g. The remaining mice were used to prepare diabetic mice model. The diabetic mice were randomly divided into five groups: model group, positive drug group (metformin hydrochloride, 300mg / kg), low sugar content polysaccharides group (200mg / kg), middle dose group (400mg / kg) (800 mg / kg). The blood glucose of mice was measured at the first and the second week after administration, and the contents of malondialdehyde (MDA) and total superoxide dismutase (T-SOD) in the serum and liver were measured and compared. Results The maximum dosage of polysaccharides was 9.596g / kg. The body weight of the model group, the positive drug group, the low, medium and high dose sugar group before and after the second week, the fourth week after administration were lower than those of the blank control group, with significant difference (P < 0.05). The fasting blood glucose in the positive group was lower than that in the model group at the second week and the fourth week after the administration, the difference was statistically significant (P <0.05). The fasting blood glucose in the high-dose group was higher than that in the model group and the low and medium-dose groups were significantly lower (P <0.05). There was no significant difference of T-SOD in serum and liver of mice (P> 0.05). The MDA level in serum and liver of the model group was higher than that of the blank control group, the differences were statistically significant (P <0.05). The content of MDA in serum and liver of the positive drug group and the low, medium and high dose group of polysaccharides was lower than that of the model group, the difference was statistically significant (P <0.05). The malondialdehyde (MDA) content in the livers of the high-dose polysaccharides group was lower than those in the low-dose and low-dose groups (P <0.05). Conclusion Polysaccharide polysaccharide has a potential hypoglycemic effect, and its hypoglycemic mechanism may be related to oxidative stress.