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30只小鼠采用盲肠结扎穿刺法制作脓毒症模型，腹腔注射15 mg/kg亚甲蓝或等渗盐水。观察肝组织病理改变，检测肝巨噬细胞频数变化和肝M1、M2型炎症因子表达情况，检测脂多糖刺激后巨噬细胞炎症因子表达水平。结果显示MB组小鼠的肝病理学损伤明显减轻（n P 0.05）；MB促进肝M2型炎症因子表达升高（ n P < 0.05）、巨噬细胞M2型炎症因子表达增多（ n P < 0.05）。MB可通过诱导巨噬细胞向M2型极化从而发挥预防脓毒症肝损伤的作用。n “,”Thirty mice were used to establish a sepsis model with cecal ligation and puncture. 15 mg/kg methylene blue or isotonic saline were injected intraperitoneally to observe liver tissue pathological changes. Changes in macrophage frequency and expressional condition of M1 and M2-type hepatic inflammatory factors were detected. After LPS stimulation, the expression level of macrophage inflammatory factor were detected. The results showed that the pathological liver injury was significantly reduced in the MB mice group (n P 0.05). MB elevation had promoted the expression of M2-type hepatic inflammatory factor ( n P < 0.05) and macrophage inflammatory factor ( n P < 0.05). MB can play a role in preventing septic liver injury by inducing macrophages polarization to M2-type.n