论文部分内容阅读
目的:建立20(S)-原人参二醇药质体含量及包封率的测定方法。方法:采用RP-HPLC法。色谱条件:COSMO-SIL 5C18-MS-Ⅱ色谱柱(4.6 mm×250 mm,5μm);流动相甲醇-水(95∶5);流速1.0 mL.min-1;检测波长203 nm;柱温25℃;进样量50μL;采用低温超速离心法分离PPD药质体中的游离药物。结果:在上述色谱条件下豆磷脂和试剂对药物的测定无干扰,20(S)-原人参二醇在0.1~0.5 g.L-1与峰面积的线性关系良好(r=0.999 9),回收率在101.44%~103.11%,日内及日间RSD均小于2%(n=3)。结论:RP-HPLC法可用于20(S)-原人参二醇药质体含量及包封率的测定。
Objective: To establish a method for the determination of 20 (S) - protopanaxadiol protoplast and entrapment efficiency. Methods: RP-HPLC method was used. The chromatographic conditions were as follows: COSMO-SIL 5C18-MS-Ⅱ column (4.6 mm × 250 mm, 5 μm); mobile phase methanol- water (95: 5); flow rate 1.0 mL · min- ℃; injection volume 50μL; using cryogenic ultracentrifugation isolated PPD drug body free drug. Results: Under the above chromatographic conditions, the determination of soapsolipid and reagent did not interfere with the determination of the drug. The linear relationship between the concentration of 20 (S) - protopanaxadiol and 0.1 ~ 0.5 gL-1 was good (r = 0.999 9) Between 101.44% and 103.11%, intra-day and intra-day RSD were less than 2% (n = 3). Conclusion: RP-HPLC method can be used to determine the content and entrapment efficiency of 20 (S) -protopanaxadiol.