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目的:探讨日本血吸虫卵细胞凋亡现象。方法:从感染兔肝脏分离血吸虫卵,用热休克方法诱导卵细胞凋亡,用DNA凝胶电泳技术检测卵细胞凋亡情况。结果:DNA电泳结果显示经热休克处理的卵细胞DNA呈凋亡特有的“梯状”条带。结论:首次证实日本血吸虫卵细胞存在凋亡现象,外源性刺激可诱导卵细胞凋亡的发生。
Objective: To investigate the phenomenon of egg cell apoptosis in Schistosoma japonicum. Methods: Schistosoma japonicum eggs were isolated from infected rabbit liver and induced apoptosis by heat shock. Egg cell apoptosis was detected by DNA gel electrophoresis. Results: The result of DNA electrophoresis showed that the DNA of heat shock-treated egg cells was a “ladder-like” band characteristic of apoptosis. Conclusion: It is the first time to confirm the apoptosis of Schistosoma japonicum egg and the exogenous stimulation can induce the apoptosis of egg.