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目的:探讨不同持续时间低氧后运动训练(高住低练)对大鼠肝组织低氧诱导因子-1α(HIF-1α)蛋白表达的影响。方法:将60只雄性SD大鼠随机分为6组,每组10只:安静对照组不训练,不进行低氧暴露;两低氧暴露组和两高住低练组每天分别进行8小时或12小时低氧暴露(氧浓度12.6%,相当于海拔4000m);同时,训练对照组和两高住低练组每天均以25m/min的速度进行跑台训练1h,每周5天,共4周。采用免疫组织化学的方法检测各组大鼠肝组织HIF-1α的蛋白表达,并采用计算机显微图像分析系统进行HIF-1α定位及阳性表达定量分析。结果:与安静对照组相比,不论是单纯低氧暴露组、单纯训练组或者高住低练组HIF-1α蛋白表达均显著增加(P<0.05);12小时低氧暴露组HIF-1α蛋白表达灰度值比8小时稍增高,无统计学意义(P>0.05),而阳性物质表达面积和PI显著增加(P<0.05);与训练对照组比较,8小时和12小时高住低练组HIF-1α蛋白表达增加,具有统计学意义(P<0.05,P<0.01);8小时高住低练组HIF-1α蛋白表达比8小时低氧暴露组显著增加(P<0.05),12小时高住低练组HIF-1α蛋白表达比12小时低氧暴露组显著增加(P<0.05,P<0.01);与8小时高住低练组相比,12小时高住低练组HIF-1α蛋白表达显著增加(P<0.05)。结论:(1)不论单纯低氧暴露、单纯训练或者高住低练均能促进肝组织HIF-1α蛋白表达增加;(2)高住低练过程中肝组织HIF-1α蛋白表达高于单纯低氧暴露或单纯训练方式,不同持续时间低氧后运动对大鼠肝组织HIF-1α蛋白表达的影响不同。
Objective: To investigate the effect of different duration hypoxic training exercises (Living High and Training Low) on the expression of hypoxia inducible factor-1α (HIF-1α) in rat liver tissue. Methods: Sixty male Sprague-Dawley rats were randomly divided into 6 groups with 10 rats in each group. The rats in the quiet control group were not trained and were not exposed to hypoxia. The two hypoxic exposure groups and the two live- 12 hours hypoxia exposure (12.6% oxygen, equivalent to an altitude of 4000m); the same time, training control group and two high living low training unit every day at 25m / min speed treadmill training 1h, 5 days a week, a total of 4 week. Immunohistochemistry was used to detect the protein expression of HIF-1α in liver tissue of each group. The localization of HIF-1α and the quantitative expression of HIF-1α were analyzed by computerized microscopic image analysis system. Results: Compared with the control group, the expression of HIF-1αprotein increased significantly (P <0.05) in both the hypobaric exposure group and the high-performing low-training group (P> 0.05), while the positive expression area and PI were significantly increased (P <0.05). Compared with the training control group, the expression level at 8 hours and 12 hours was significantly lower than that in the training control group (P <0.05, P <0.01). The expression of HIF-1αprotein in high-living-low training group increased significantly (P <0.05) and the expression of HIF-1αprotein in 8 hours HIF-1αprotein expression in HHGH group was significantly higher than that in HHH group (P <0.05, P <0.01) 1α protein expression was significantly increased (P <0.05). Conclusions: (1) HIF-1αprotein expression in liver tissue can be promoted by both simple training and high living-low training regardless of exposure to hypoxia alone. (2) HIF-1αprotein expression in liver tissue is higher than that in simple hypoxia Oxygen exposure or training alone, different duration of hypoxia after exercise on rat liver tissue HIF-1αprotein expression.