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丙酮酸羧化酶（ Ｐ Ｅ Ｐ）是控制油菜蛋白质／油脂含量比例的一个关键酶⒚本研究用 Ｐ Ｃ Ｒ 法扩增出了 Ｐ Ｅ Ｐ 基因片段，并将其 克隆到 ｐ Ｂ Ｓ Ｓ Ｋ＋ 的 Ｓｍ ａ Ⅰ位点⒚ Ｄ Ｎ Ａ 序列分析表 明克隆片段 的长度为 ５３０ｂｐ，其序列与报道序列相同⒚将该 Ｐ Ｅ Ｐ基因 片段反向插入 ｐ Ｉ Ｇ１２１ 质粒，构建了带 Ｐ Ｅ Ｐ 反义基因的超级双元载体并进行油菜的转化，目前已获得转基因植株⒚
Pyruvate carboxylase (P E P) is a key enzyme that controls the ratio of protein / lipid content in rapeseed. In this study, the P E P gene fragment was amplified by P C R method and cloned into p B S S K + The SmA Ⅰ site ⒚ D N A sequence analysis showed that the length of the cloned fragment was 530 bp with the same sequence as the reported sequence. The P E P gene fragment was inserted into the pI G121 plasmid reversely to construct a plasmid with P E P antisense Gene super binary vector and the transformation of rapeseed, has been obtained transgenic plants ⒚