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目的:建立HPLC-DAD法同时测定中药苦参中氧化槐醇碱、氧化苦参碱、槐定碱、氧化槐果碱、槐醇、苦参碱及槐果碱七种生物碱的含量。方法:Agilent Zorbax C_(18)色谱柱(150 mm×4.6 mm,5μm),流动相为10 mmol·L~(-1)醋酸铵水溶液(0.05%氨水,p H=9.0)(A)-20 mmol·L~(-1)醋酸铵的甲醇-乙腈(1∶2)混合溶液(B),梯度洗脱,流速:1.0 ml·min~(-1),检测波长:220 nm,柱温:30℃。结果:氧化槐醇碱、氧化苦参碱、槐定碱、氧化槐果碱、槐醇、苦参碱及槐果碱的质量浓度分别在0.093~1.860μg(r=0.999 6)、0.530~10.600μg(r=0.999 7)、0.062~1.240μg(r=0.999 8)、0.281~5.620μg(r=0.999 9)、0.026~0.520μg(r=0.999 8)、0.036~0.720μg(r=0.999 7)及0.032~0.640μg(r=0.999 6)内与峰面积呈良好的线性关系。氧化槐醇碱、氧化苦参碱、槐定碱、氧化槐果碱、槐醇、苦参碱及槐果碱的平均加样回收率分别为97.5%、98.2%、99.0%、99.4%、99.2%、98.2%和98.7%,RSD分别为1.18%、0.92%、1.43%、1.04%、0.81%、0.43%和0.88%(n=6)。结论:该方法简便、准确、重复性好,可用于苦参药材多成分的质量控制研究。
OBJECTIVE: To establish a HPLC-DAD method for simultaneous determination of seven alkaloids, including oxybenzone, oxymatrine, sophoridine, oxycinnamine, sophyl alcohol, matrine and sophocarpine in Sophora flavescens. METHODS: Agilent Zorbax C_ (18) column (150 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of 10 mmol·L -1 ammonium acetate aqueous solution (0.05% The gradient elution was carried out at a flow rate of 1.0 ml · min ~ (-1), the detection wavelength was 220 nm, the column temperature was: 30 ° C. Results: The concentrations of oxybenzone, oxymatrine, sophoridine, oxysophocarpine, sophora alcohol, matrine and sophocarpine were respectively in the range of 0.093-1.860 μg (r = 0.999 6) and 0.530-10.600 (r = 0.999 8), 0.036-0.720 μg (r = 0.999 7), 0.062-1.240 μg (r = 0.999 8), 0.281-5.620 μg (r = 0.999 9), 0.026-0.520 μg ) And 0.032 ~ 0.640μg (r = 0.999 6) and the peak area showed a good linear relationship. The average recoveries of oxybenzone, oxymatrine, sophoridine, oxysophocarpine, sophora alcohol, matrine and sophocarpine were 97.5%, 98.2%, 99.0%, 99.4%, 99.2 %, 98.2% and 98.7%, respectively. The RSDs were 1.18%, 0.92%, 1.43%, 1.04%, 0.81%, 0.43% and 0.88%, respectively (n = 6). Conclusion: This method is simple, accurate and reproducible. It can be used to study the quality control of multiple components of Sophora flavescens.