应用同位素标记前体掺入和流式细胞分析的方法,研究CP对体外培养K562细胞的直接作用。发现CP可显著抑制K562细胞的DNA和RNA合成,其抑制程度随CP剂量增加而增强,并能导致K562细胞的不平衡生长;还发现CP对K562细胞的直接作用没有明显改变K562细胞在细胞周期中的分布。表明CP对K562细胞具有直接毒性作用,这种毒性作用是非细胞周期特异性的。提示在CP胸膜腔内注射治疗恶性胸水的机理中可能存在着CP对肿瘤细胞的直接毒性作用。 The direct effect of CP on K562 cells was studied by isotope labeled precursor incorporation and flow cytometry. Found that CP could significantly inhibit the synthesis of DNA and RNA in K562 cells, the inhibition increased with the increase of CP dose, and led to the unbalanced growth of K562 cells; also found that the direct effect of CP on K562 cells did not significantly change K562 cells in the cell cycle In the distribution. This indicates that CP has a direct toxic effect on K562 cells. This toxic effect is non-cell cycle specific. Tip CP pleural cavity injection of malignant pleural effusion may exist in the mechanism of CP on the direct toxicity of tumor cells.