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研究荷叶多成分整体的肠渗透性,明确荷叶成分间吸收过程中的相互作用。采用大鼠在体单向灌流法。结果表明,荷叶中荷叶碱、去甲基荷叶碱、芦丁、异槲皮苷、紫云英苷、槲皮素、山柰酚7种单体成分的有效渗透系数(Peff)大于0.5×10~(-4)cm·s~(-1),在生物药剂学分类系统(biopharmaceutics classification system,BCS)肠渗透属性中,属高渗透性成分,而儿茶素、金丝桃苷为低渗透性成分。然而,在荷叶总提取物多成分环境下,成分的渗透行为发生了变化,对荷叶中不明确成分进行半定量分析发现,在多成分环境下,可标识的9个成分中有3个成分Peff小于0.5×10~(-4)cm·s~(-1)。在多成分环境下,7个未知成分中,3个成分属于高渗透性成分,4个成分属于低渗透性成分。该研究初步明确了荷叶中多成分整体的肠渗透性,为揭示中药多成分环境下的吸收机制奠定一定的基础。
To study the intestine permeability of the multi-component lotus leaf, and to clarify the interaction between lotus leaf components in the process of absorption. Rat in vivo one-way perfusion method. The results showed that the effective permeation coefficient (Peff) of the seven monomer components of lotus leaf, demethylation, rutin, isoquercetin, astragalin, quercetin and kaempferol was higher than 0.5 × 10 -4 cm · s -1, which is a highly permeable component in intestinal permeability of biopharmaceutics classification system (BCS), whereas catechin, hyperin For low permeability ingredients. However, under the multi-component environment of total lotus leaf extract, the osmotic behavior of the components changed. Semi-quantitative analysis of the indefinite components in the lotus leaf revealed that in the multi-component environment, three of the 9 identifiable components The composition Peff is less than 0.5 × 10 -4 cm · s -1. In the multi-component environment, of the seven unknown components, three components belong to the high permeability component and the four components belong to the low permeability component. The study initially clarified the intestine permeability of the multi-component lotus leaf and laid a foundation for revealing the absorption mechanism under the multi-component environment of traditional Chinese medicine.