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目的:比较不同产地夏枯草中抗非小细胞肺癌活性成分科罗索酸的含量差异。方法:通过四甲基偶氮唑蓝(MTT)和吖啶橙(AO)/溴化乙啶(EB),评价科罗索酸对非小细胞肺癌细胞的增殖抑制以及凋亡作用;建立高效液相色谱法,比较14个产地夏枯草中科罗索酸的含量差异。结果:科罗索酸抑制SPC-A-1人非小细胞肺癌的生长增殖(IC50=1.05×10-6mol/L),并且诱导SPC-A-1细胞凋亡。14个产地夏枯草中科罗索酸的含量差异较大,山西产地的含量最高,河南-2产地的含量最低。结论:科罗索酸为夏枯草中的抗非小细胞肺癌的活性成分,不同产地夏枯草的科罗索酸含量差异较大。
Objective: To compare the content of corosolic acid in anti-non-small cell lung cancer active ingredients from Prunella vulgaris in different areas. Methods: MTT, AO / EB were used to evaluate the inhibitory effect of corosolic acid on the proliferation and apoptosis of non-small cell lung cancer cells. A high efficiency Liquid chromatography, comparison of 14 areas of Prunella in the content of corosolic acid. RESULTS: Corosolic acid inhibited the proliferation and proliferation of SPC-A-1 human non-small cell lung cancer (IC50 = 1.05 × 10-6 mol / L) and induced the apoptosis of SPC-A-1 cells. The contents of corosolic acid in Prunella vulgaris were significantly different among 14 producing areas, the content in Shanxi origin was the highest, and the content in Henan -2 was the lowest. CONCLUSION: Corosolic acid is the active component of anti-non-small cell lung cancer in Prunella vulgaris, and the corosolic acid content of Prunella vulgaris differs greatly from each other.