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为探讨大黄泻下作用与肠道 5 -HT及其受体的关系 ,将成年SD大鼠 2 0只随机分为灌服大黄组 (A组10只 )和对照组 (B组 10只 )。采用免疫组化和医学图像分析系统相结合的方法 ,测定各组大鼠十二指肠 5 -HT细胞的数目、面积、平均灰度、细胞内 5 -HT含量及 5 -HT受体的含量。结果发现A组大鼠十二指肠 5 -HT细胞的数目 (10 8 80± 5 2 6 )、面积 (30 18± 2 18) μm2 、5 -HT含量 (0 340 9± 0 0 30 8)AU、5 -HT受体含量 (0 3188± 0 0 312 )AU均高于B组 (P <0 0 1) ,平均灰度 (12 3 38± 3 94 )较B组降低 (P <0 0 1)。结果表明大黄的泻下作用可能与肠道 5 -HT及其受体含量的增高有密切关系。
To investigate the relationship between rhubarb diarrhea and intestinal 5-HT and its receptors, 20 adult SD rats were randomized to receive either rhubarb (Group A) or control (Group B). The number, area, average gray scale, intracellular 5-HT content, and 5-HT receptor content of 5-HT cells in the duodenum of rats in each group were determined using a combination of immunohistochemistry and a medical image analysis system. . The results showed that the number of 5-HT cells in the duodenum of group A rats (10 8 80 ± 5 2 6 ), area (30 18 ± 2 18) μm 2 and 5-HT content (0 340 9 ± 0 0 30 8) The AU, 5-HT receptor content (0 3188 ± 0 0 312) AU were higher than B group (P <0 01), and the average gray scale (12 3 38 ± 3 94) was lower than that of B group (P <0 0 1). The results indicate that the purgative effect of rhubarb may be closely related to the increase of 5-HT and its receptor content in the intestine.