论文部分内容阅读
目的观察肝动脉As2O3碘油栓塞对兔肝移植瘤凋亡、增生细胞核抗原(PCNA)表达及肝功能的影响。方法40只家兔肝内肿瘤种植后2周,随机分为5组,经肝动脉插管分别给予不同处理,实验设生理盐水灌注组(A组)、As2O3灌注组(B组)、单纯碘油栓塞组(C组)、阿霉素碘油栓塞组(D组)及As2O3+碘油栓塞组(E组),As2O3的用量为2 mg/kg。治疗前3 d,治疗后4、7 d,耳缘静脉取血,测定部分肝功能指标。采用原位末端标记法检测肿瘤细胞的凋亡指数,免疫组化方法测定肿瘤细胞增生细胞核抗原的表达。结果治疗后4 d,栓塞治疗组AST、ALT上升,治疗后7 d,肝功能渐趋正常,阿霉素(ADM)碘油栓塞治疗组AST、ALT水平高于其它组。各组的凋亡指数和增殖指数分别为1.53±0.42、1.82±0.41、2.66±0.54、2.91±0.32、3.44±0.65和60.8±15.5、55.9±14.8、42.4±11.2、40.6±8.8、28.5±5.7,两者存在负相关。结论As2O3比ADM对正常肝组织的毒性低。As2O3碘油栓塞治疗后残余肿瘤的凋亡增加,肿瘤细胞的增殖能力下降。
Objective To observe the effect of hepatic arterial As2O3 lipiodol embolization on hepatic tumor cell apoptosis, expression of proliferating cell nuclear antigen (PCNA) and liver function in rabbits. Methods Forty rabbits with intrahepatic tumor were randomly divided into 5 groups after 2 weeks of implantation. The hepatic artery was cannulated to give different treatment. The experimental group was divided into three groups: normal saline group (group A), As2O3 group (group B) (C group), doxorubicin lipiodol embolization group (D group), and As2O3 + lipiodol embolization group (E group). The dosage of As2O3 was 2 mg / kg. 3 d before treatment, 4 d and 7 d after treatment, blood was collected from the ear vein to determine some indexes of liver function. The apoptosis index of tumor cells was detected by in situ end labeling and the expression of tumor cell proliferating cell nuclear antigen was detected by immunohistochemistry. Results At 4 days after treatment, the AST and ALT levels increased in the embolization group. The liver function gradually became normal on the 7th day after treatment. The levels of AST and ALT in the embolization group were higher than those in the other groups. The apoptosis index and proliferation index in each group were 1.53 ± 0.42, 1.82 ± 0.41, 2.66 ± 0.54, 2.91 ± 0.32, 3.44 ± 0.65 and 60.8 ± 15.5, 55.9 ± 14.8, 42.4 ± 11.2, 40.6 ± 8.8, 28.5 ± 5.7 , There is a negative correlation between the two. Conclusion As2O3 is less toxic to normal liver tissue than ADM. As2O3 lipiodol embolization treatment of residual tumor increased, the proliferation of tumor cells decreased.