利用TAMⅢ等温微量量热仪研究不同浓度的石油污染对土壤微生物活性的影响。对大港油田不同石油污染程度的土壤样品(3683-5-1、西51-5、西15-14和8-13-6H-1)以及实验室配置的短时间内石油污染土壤(空白、0.5%、1.5%、2.5%和4.0%)进行了微量热检测。含油量升高,总放热量升高,生长速率常数降低,峰值功率先升高后降低,达到最大峰值功率的时间随着含油量的升高而延长。结果表明,石油污染会影响土壤微生物的活性,并且在低浓度时促进微生物的生长,石油浓度高时会抑制土壤中微生物的生长代谢。 The effect of different concentrations of petroleum pollution on soil microbial activity was studied by TAMⅢ isothermal microcalorimeter. Soil samples (3683-5-1, 51-5, West 15-14 and 8-13-6H-1) with different levels of petroleum contamination in Dagang oilfield and petroleum contaminated soil (blank, 0.5 %, 1.5%, 2.5% and 4.0%). Oil content increased, the total exothermic increased, the growth rate constant decreased, the peak power first increased and then decreased, reaching the maximum peak power of the time with the oil content increased. The results show that oil pollution can affect the activity of soil microorganisms, and promote the growth of microorganisms at low concentration. When the oil concentration is high, it can inhibit the growth and metabolism of soil microorganisms.