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目的研究蛹虫草对急性一氧化碳(CO)中毒大鼠的脑保护作用及其机制。方法 24只成年雄性SD大鼠随机分为对照组(C组)、CO模型组(CO组)和蛹虫草干预组(Cordyceps组),每组8只。采用吸入法制作急性CO中毒模型,造模后14 d为时相点,应用Morris水迷宫试验检测大鼠平均潜伏期;根据Brailowsky描述的神经学评分方法对神经功能缺损进行症状评估;对海马组织行HE及突触素免疫组化染色;采用Western Blot方法检测各组大鼠海马组织NF-κB p65蛋白的表达。结果水迷宫测试提示蛹虫草干预组平均潜伏期短于CO模型组(P<0.05)。蛹虫草干预组神经功能缺失症状明显减轻(P<0.05);HE染色表明蛹虫草对CO中毒后海马有明显的保护作用;与CO模型组相比,蛹虫草能促进突触素的生成、降低海马组织NF-κB p65蛋白含量。结论蛹虫草对大鼠急性CO中毒脑损伤有保护作用,其机制可能是通过上调突触素的表达、降低NF-κB p65蛋白水平而发挥作用。
Objective To study the protective effects of Cordyceps militaris on acute carbon monoxide (CO) poisoning in rats and its mechanism. Methods Twenty-four adult male Sprague-Dawley rats were randomly divided into control group (C group), CO model group (Cox group) and Cordyceps group (Cordyceps group). The model of acute CO poisoning was established by inhalation and the time point was 14 days after modeling. Morris water maze test was used to detect the average latency of rats. The neurological deficits were evaluated according to the neurological score method described by Brailowsky. HE and synaptophysin immunohistochemical staining. Western blot was used to detect the expression of NF-κB p65 protein in hippocampus of rats in each group. Results The water maze test suggested that the average incubation period of Cordyceps militaris group was shorter than that of CO model group (P <0.05). Cordyceps Militaris intervention group, neurological deficit symptoms were significantly reduced (P <0.05); HE staining showed that Cordyceps militaris significantly inhibited the hippocampus after CO poisoning; compared with the CO model group, Cordyceps militaris can promote the formation of synaptophysin and reduce The content of NF-κB p65 protein in hippocampus tissue. Conclusion Cordyceps militaris has a protective effect on brain injury induced by acute CO intoxication in rats. The mechanism may be through up-regulating the expression of synaptophysin and decreasing the level of NF-κB p65 protein.