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[目的]探讨二氧化碳(CO2)气腹对膀胱癌细胞黏附和侵袭能力的影响。[方法]体外培养膀胱癌T24细胞,分别以5、10、15mmHg不同CO2压力和1个大气压的空气作用于癌细胞2、4、6h,以MTT法和Boyden侵袭小室法检测细胞体外黏附能力和侵袭能力改变。[结果]同一时间点不同压力组与对照组比较,平均吸光度值(A值)差异均有统计学意义(2h:F=33.271,P=0.000;4h:F=26.053,P=0.000;6h:F=39.831,P=0.000)。相同压力下,随作用时间增长,A值逐渐增大(5mmHg:F=11.926,P=0.000;10mmHg:F=14.117,P=0.000;15mmHg:F=20.652,P=0.000)。Boyden小室侵袭实验显示,5、10mmHgCO2压力作用4h内,穿过滤膜的细胞数目与对照组比较无显著性差异,但15mmHg压力作用6h时,穿过滤膜的细胞数目明显增多(P<0.05)。[结论]CO2气腹环境可促进体外培养的膀胱癌细胞侵袭转移,可能与CO2气腹作用的压力和时间密切相关。
[Objective] To investigate the effect of carbon dioxide (CO2) pneumoperitoneum on the adhesion and invasion ability of bladder cancer cells. [Method] The bladder cancer T24 cells were cultured in vitro. The cells were exposed to air pressure of 5, 10 and 15 mmHg under different CO2 pressures and 1 atm for 2, 4 and 6 hours respectively. The in vitro cell adhesion ability Invasive ability changes. [Result] The average absorbance value (A value) in different pressure groups at the same time point was significantly different from the control group (2h: F = 33.271, P = 0.000; 4h: F = 26.053, P = F = 39.831, P = 0.000). Under the same pressure, the value of A gradually increased with time increasing (5mmHg: F = 11.926, P = 0.000; 10mmHg: F = 14.117, P = 0.000; 15mmHg: F = 20.652, P = 0.000). Boyden chamber invasion assay showed that there was no significant difference in cell number between 5 and 10 mmHgCO2 for 4 h compared with that of the control group. However, the number of cells passing through the filter increased significantly (P <0.05) at 15 mmHg pressure for 6 h. [Conclusion] CO2 pneumoperitoneum can promote the invasion and metastasis of bladder cancer cells cultured in vitro, which may be closely related to the pressure and time of CO2 pneumoperitoneum.