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目的 :观察芪丹通脉片 (QDTMT)对实验性动脉粥样硬化 (AS)大鼠动脉壁匀浆中细胞间黏附分子 1 (ICAM 1 )和血管细胞黏附分子 1 (VCAM 1 )表达的影响 ,探讨QDTMT的抗AS机制 .方法 :将健康雄性SD大鼠 72只按体质量随机分为6组 ,每组 1 2只 :模型组 (model)、空白对照组 (control)、阳性对照辛伐他汀组 (simvastatin)、QDTMT低剂量组 (QDTMTL)、QDTMT中剂量组 (QDTMTM)、QDTMT高剂量组 (QDTMTH) .采用高脂饮食配合口服维生素D3建立大鼠AS模型 ,各组动物ig给药 .采用半定量RT PCR的方法检测各组动物动脉壁匀浆中ICAM 1和VCAM 1基因的表达 ,分析造模及各药物组I CAM 1和VCAM 1基因表达的变化 .结果 :与空白对照组相比 ,模型组ICAM 1和VCAM 1基因的表达明显增加 (P <0 .0 1 ) ;与模型组相比 ,辛伐他汀组及各中药组均能减少ICAM 1和VCAM 1基因的表达 (P <0 .0 1 - 0 .0 5 ) ,且QDTMTH的作用明显优于QDTMTL(P <0 .0 5 ) .结论 :QDTMT能下调实验性AS大鼠动脉壁ICAM 1和VCAM 1基因的表达 ,这可能是QDTMT抗AS的机制之一 .
Objective: To observe the effect of QDTMT on the expression of intercellular adhesion molecule 1 (ICAM 1) and vascular cell adhesion molecule 1 (VCAM 1) in artery wall homogenates of experimental atherosclerotic rats. Investigate the anti-AS mechanism of QDTMT. Methods: 72 healthy male SD rats were randomly divided into 6 groups according to their body mass, 12 in each group: model group, control group, and positive control group The simvastatin, QDTMT low-dose group (QDTMTL), QDTMT middle-dose group (QDTMTM), and QDTMT high-dose group (QDTMTH) were used to establish a rat AS model with high-fat diet and oral vitamin D3. The semiquantitative RT PCR method was used to detect the expression of ICAM 1 and VCAM 1 genes in the arterial wall homogenate of each group of animals. The expression of ICAM 1 and VCAM 1 genes in the model group and various drug groups were analyzed. Results: Compared with the blank control group. Compared with the control group, the expression of ICAM 1 and VCAM 1 genes in the model group was significantly increased (P < 0.01). Compared with the model group, both the simvastatin group and each TCM group were able to reduce the expression of ICAM 1 and VCAM 1 genes ( P <0 .0 1 - 0 .0 5 ), and the effect of QDTMTH is significantly better than that of QDTMTL (P <0 . 0 5 ). Conclusion: QDTMT It can down-regulate the expression of ICAM 1 and VCAM 1 gene in the arterial wall of experimental rats, which may be one of the mechanisms of QDTMT anti-AS.