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目的研究红霉素和氨溴索分别联合环丙沙星雾化吸入对铜绿假单胞菌成熟生物膜的干预效果。方法平板法培养成熟铜绿假单胞菌生物膜;微量肉汤稀释法测量红霉素和环丙沙星的最低抑菌浓度;制作气管插管铜绿假单胞菌生物膜感染模型;平板计数法计算红霉素、氨溴索分别联合环丙沙星对生物膜菌落数的影响;日本岛津紫外-可见光分光光度计UV1700测铜绿假单胞菌菌液的A值;石蜡切片HE染色定性观察肺组织的炎症情况;扫描电镜定性观察各处理组的生物膜结构变化。结果各处理组干预7 d后肺组织细菌菌落计数(×104CFU/m l):干预组分为:生理盐水对照,氨溴索,红霉素,红霉素联合环丙沙星,氨溴索联合环丙沙星,各组分别为139.250±42.0162、101.625±40.4190、109.625±33.4747、57.750±37.8295和22.250±17.3184,前3组与后2组对比差异均有显著性(P<0.05),前3组之间对比差异没有显著性(P>0.05),后2组对比差异有显著性(P<0.05)。导管生物膜细菌菌落计数(×104CFU/m l):5组分别为170.000±48.3263、127.625±39.0163、133.500±33.6876、70.375±35.7768和38.125±19.1045,结论和肺组织菌落计数是一致的。导管生物膜电镜观察:第1组导管内表面均有较厚基质覆盖,2、3组减少不明显,而联合用药组导管内表面生物膜明显减少,其中第5组效果更好。结论氨溴索与红霉素分别联合环丙沙星雾化吸入在控制导管生物膜和呼吸系统相关感染均具有显著效果,其中氨溴索联合环丙沙星疗效更好。
Objective To study the effect of inhalation of erythromycin and ambroxol combined with ciprofloxacin on mature biofilm of Pseudomonas aeruginosa. Methods The plate-culture method was used to culture the mature Pseudomonas aeruginosa biofilm. The minimal inhibitory concentrations of erythromycin and ciprofloxacin were measured by the broth microdilution method. The biofilm-infected model of tracheal intubation Pseudomonas aeruginosa was established. The effect of erythromycin and ambroxol combined with ciprofloxacin on the number of biofilm colonies was calculated respectively. The value of A was determined by UV1700 UV-Vis spectrophotometer in Japan. The qualitative observation of paraffin sections by HE staining Inflammation of lung tissue; scanning electron microscopy qualitative observation of the biofilm structure changes in each treatment group. Results Bacterial colonies count (× 104CFU / ml) in lung tissue of each treatment group after intervention for 7 d was as follows: the intervention group consisted of saline control, ambroxol, erythromycin, erythromycin combined with ciprofloxacin, ambroxol Ciprofloxacin, respectively, the groups were 139.250 ± 42.0162,101.625 ± 40.4190,109.625 ± 33.4747,57.750 ± 37.8295 and 22.250 ± 17.3184, the difference between the first three groups and the latter two groups were significant (P <0.05), the first three There was no significant difference between the two groups (P> 0.05). The difference between the two groups was significant (P <0.05). Bacterial colony counts of catheter biofilms (104 CFU / ml): 170.000 ± 48.3263, 127.625 ± 39.0163, 133.500 ± 33.6876, 70.375 ± 35.7768 and 38.125 ± 19.1045 for the 5 groups, respectively, consistent with lung tissue colony counts. Catheter biofilm electron microscopy: the first group of catheter surface thicker substrate coverage, 2,3 group was not significantly reduced, while the combined group of catheter surface biofilm significantly reduced, of which the fifth group better. Conclusion Ambroxol and erythromycin combined with inhalation of ciprofloxacin have significant effects on the control of biofilm and respiratory-related infections, and ambroxol combined with ciprofloxacin has better efficacy.