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采用间接免疫荧光染色、流式细胞仪、激光扫描共聚焦显微镜、Ｎｏｒｔｈｅｒｎｂｌｏｔ及Ｗｅｓｔｅｒｎｂｌｏｔ分析研究分化抗原６Ａ８在人淋巴细胞的表达。流式细胞仪分析显示，单抗６Ａ８与所检测的Ｂ细胞株３Ｄ５、ＣＥＳＳ和Ｄａｕｄｉ有反应，与人组织细胞瘤细胞株Ｕ９３７也有反应，与慢性髓性白血病细胞株Ｋ５６２及所检测的Ｔ细胞株Ｊｕｒｋａｔ和Ｐｅｅｒ不反应。Ｎｏｒｔｈｅｒｎｂｌｏｔ检测支持上述现象，３Ｄ５和Ｕ９３７细胞ｍＲＮＡ转录较丰富，ＣＥＳＳ细胞次之，而Ｊｕｒｋａｔ与Ｐｅｅｒ细胞的ｍＲＮＡ转录很少。激光扫描共聚焦显微镜观察显示，单抗６Ａ８识别的分化抗原见于３Ｄ５细胞浆和胞膜。可见在人Ｂ细胞株３Ｄ５，分化抗原６Ａ８不仅表达于胞膜，也表达于胞浆。
Indirect immunofluorescence staining, flow cytometry, laser scanning confocal microscopy, Northern blot and Western blot analysis of differentiated antigen 6A8 expression in human lymphocytes. Flow cytometry analysis showed that monoclonal antibody 6A8 reacted with the detected B cell lines 3D5, CESS and Daudi, and also reacted with the human histocytic tumor cell line U937. Compared with the chronic myeloid leukemia cell line K562 and the detected T cells Strains Jurkat and Peer do not react. Northernblot assay supports the above phenomenon, 3D5 and U937 cells mRNA transcription more abundant, CESS cells followed, and Jurkat and Peer cells mRNA transcription is very small. Laser scanning confocal microscopy showed that the differentiated antigen recognized by mAb 6A8 was found in 3D5 cytoplasm and plasma membrane. Can be seen in the human B cell line 3D5, differentiation antigen 6A8 is not only expressed in the membrane, but also expressed in the cytoplasm.