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目的 探讨 PHC患者 PBMC AFP m RNA表达与微转移的相关性。方法 应用巢式 RT- PCR检测 10 2例PHC、2 2例转移性肝癌及其他恶性肿瘤 ,16例慢性乙型肝炎、2 2例肝硬化和 4 0例献血员中 PBMC AFP m RNA表达水平。结果 AFP m RNA在献血员、转移性肝癌及其他恶性肿瘤、慢性乙型肝炎患者 PBMC中均为阴性 ;在 PHC组 PBMC中 AFP m RNA的检出率 (5 6 .86 % ,5 8/10 2 )明显高于肝硬化组 (9.0 9% ,2 /2 2 ,P<0 .0 5 )。 PBMC AFP m RNA的表达与临床分期、门静脉癌栓、肝内转移、远处转移明显相关 (<0 .0 5 ) ,而与肿瘤直径、数目及血清 AFP浓度无明显相关 (>0 .0 5 )。结论 巢式 RT- PCR检测 PHC患者 PBMC AFP m RNA是一种早期发现 PHC血道播散的可靠而又敏感的实验方法。
Objective To investigate the correlation between AFP m RNA expression and micrometastasis in PBMC of PHC patients. Methods AFP m RNA was detected by nested RT-PCR in 102 cases of PHC, 22 cases of metastatic liver cancer and other malignant tumors, 16 cases of chronic hepatitis B, 22 cases of liver cirrhosis and 40 cases of blood donors. Results AFP m RNA was negative in PBMC of blood donors, metastatic liver cancer and other malignant tumors and chronic hepatitis B patients. The detection rate of AFP m RNA in PBMC of PHC group (56.86%, 58.10% 2) was significantly higher than that in cirrhosis (9.0 9%, 2/2 2, P <0.05). The expression of PBMC AFP m RNA was significantly associated with clinical stage, portal vein tumor thrombus, intrahepatic metastasis and distant metastasis (<0.05), but not with tumor diameter, number and serum AFP concentration (> 0.05) ). Conclusion The detection of AFP m RNA in PBMC of PHC patients by nested RT-PCR is a reliable and sensitive experimental method for detecting the dissemination of PHC in the early stage.