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我们过去曾报道束缚应激可使大鼠和小鼠血清中产生一种150及370kd的大分子蛋白质,可明显抑制ConA诱导的正常小鼠淋巴细胞转化(简称血清抑制因子)。对其产生部位也作过初步研究,证明它可能与淋巴细胞中的T细胞有关。本工作进一步证明其主要淋巴结和脾脏等淋巴组织产生而释放到血中。 实验动物为SD大鼠,雌雄不拘,体重180~200g,束缚16~20小时(应激方法与以前的实验相同)。无菌条件下摘取胸腺,肠系膜集合淋巴结、脾脏、全脑、大腿骨髂肌。0℃下在0.005mol/L HCI中匀浆,4℃离心(2000g)30分钟,取上清液,冷冻抽干后,以RPMI 1640复溶,观察各组织提取液对ConA诱导正常小鼠淋巴细胞转化的影响。
We have reported in the past that restraint stress can produce a 150 and 370 kd macromolecular protein in the serum of rats and mice, which can significantly inhibit ConA-induced normal mouse lymphocyte transformation (abbreviated as serum suppressor factor). A preliminary study of the site of its production has also been conducted to prove that it may be related to T cells in lymphocytes. This work further proves that lymph nodes such as lymph nodes and spleen are produced and released into the blood. The experimental animals were SD rats, either male or female, weighing 180-200 g, bound for 16-20 hours (the stress method was the same as previous experiments). Under aseptic conditions, the thymus, mesenteric lymph nodes, spleen, whole brain, and femoral tibial muscles were removed. Homogenate in 0.005 mol/L HCI at 0°C, centrifugation (2000g) for 30 minutes at 4°C, take the supernatant, freeze-dry, freeze-dry, reconstitute in RPMI 1640, observe each tissue extract on ConA-induced normal mouse lymphatic The effect of cell transformation.