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目的通过观察慢性乙型肝炎(慢乙肝)患者尿中转化生长因子β1(TGF-β1)表达水平,探讨尿TGF-β1水平在慢乙肝和乙肝后肝硬化(肝硬化)中的意义。方法用酶联免疫吸附试验(ELISA)检测63例慢乙肝(CHB)、肝硬化(LC)患者和20名健康体检者(对照组)尿TGF-β1浓度;结果肝硬化组尿TGF-β1水平明显高于对照组(P<0.01);肝硬化组中代偿期肝硬化组与失代偿期肝硬化组均明显高于对照组(均P<0.01);且尿TGF-β1水平与肝纤维化指标Ⅳ型胶原、透明质酸水平呈正相关(P<0.05)。结论尿TGF-β1在肝硬化患者中随病情严重程度加重其水平上升,提示尿TGF-β1水平有助于肝细胞受损程度的判断以及肝硬化诊断。
Objective To investigate the expression of transforming growth factor-β1 (TGF-β1) in urine of patients with chronic hepatitis B (chronic hepatitis B) and to explore the significance of urinary TGF-β1 in chronic hepatitis B and post-hepatitis B cirrhosis (cirrhosis). Methods Urinary TGF-β1 in 63 patients with chronic hepatitis B (CHB), cirrhosis (LC) and 20 healthy controls (control group) was detected by enzyme-linked immunosorbent assay (ELISA) (P <0.01). The levels of TGF-β1 in urine of cirrhotic patients with decompensated cirrhosis and decompensated cirrhosis were significantly higher than those of the control group (all P <0.01) Fibrosis index type Ⅳ collagen, hyaluronic acid level was positively correlated (P <0.05). Conclusion Urinary TGF-β1 in patients with cirrhosis aggravated with the severity of its increased level, suggesting that urinary TGF-β1 levels contribute to the determination of liver cell damage and cirrhosis diagnosis.