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根据胶元蛋白酶能水解胶元蛋白而对其它蛋白(如纤维蛋白、球蛋白等)不起作用的特点,以及利用其不但能水解烫伤结节中心的变性胶元,并能水解边缘区域的半坏死组织的性质,我们用胶元蛋白酶对实验动物进行了烫伤的清创脱痂作用试验。实验方法 1.模型制备:用豚鼠直接水烫法。选择体重500～650克左右的健康雄性豚鼠8只,剃毛后用苯巴比妥(10毫克/公斤)腹腔注射麻醉后,将腹部双侧离开脊骨2厘米处分别按至盛有80℃热水之烧杯(杯口直径为4.5厘米)上,直接烫伤20秒钟。提起动物,立即以96％乙醇不断擦烫伤部位,使之迅速冷却,所造成
According to collagen cell protease can hydrolyze collagen protein and other proteins (such as fibrin, globulin, etc.) does not work characteristics, and use not only to hydrolyze the center of the scalded nodules gelatin yuan, and can hydrolyze the edge of the half The nature of the necrotic tissue, we use collagenase protease on scalded animals scored debridement test. Experimental methods 1. Model Preparation: Guinea pigs with direct water hot method. Eight healthy male guinea pigs weighing between 500 and 650 grams were selected. After shaving, they were anesthetized with phenobarbital (10 mg / kg) intraperitoneally, and the abdomen were removed 2 cm apart from the spine on both sides to 80 ° C Hot water beaker (cup diameter of 4.5 cm), direct burns for 20 seconds. Lift the animal and immediately wipe the area with 96% ethanol to quickly cool it, resulting in