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辐射或某些化学因子导致的成年鼠肝脏潜在损害可在刺激增殖(如部分切除)后通过生化和细胞学改变显示出来。在再生肝中最明显的改变是DNA合成和有丝分裂的抑制及染色体畸变率的增加。在先前受到医疗或事故照射的人员遇到导致肝细胞坏死或丧失的情况下估计肝脏再生能力时,关于再生过程的详细知识具有重要意义。作者给Wistar大鼠全身照射2.9或5.8Gy X线TURT-250后,立即切除2/3肝脏(对照组只切肝不照射)并在切除后18小时至21天中的规定时间活杀动物,检查~3H-胸腺核苷掺入量、肝内DNA含量、肝细胞核DNA含量、分裂指数、核计数以及双核肝细胞数。
Potential damage to adult livers from radiation or certain chemical agents can be shown by biochemical and cytological changes after stimulating proliferation (eg, partial resection). The most obvious changes in regenerative liver are DNA synthesis and inhibition of mitosis and increased chromosome aberrations. When a person previously exposed to medical or accidental radiation experiences hepatic regenerative capacity in the presence of hepatocyte necrosis or loss, detailed knowledge of the regeneration process is of great importance. After whole body irradiation of Wistar rats with 2.9 or 5.8 Gy X-ray TURT-250, the animal was immediately excised with 2/3 of the liver (the control group received no hepatic irradiation only) and the animals were sacrificed within 18 hours to 21 days after resection. The ~3H-thymidine incorporation, intrahepatic DNA content, hepatocyte nuclear DNA content, mitotic index, nuclear count, and binucleated hepatocyte number were examined.