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三碘苯甲酸钠类造影剂在放射诊断中已普遍应用。目前的统计表明,在欧美每年大约使用一千万支造影剂。虽然这些造影剂尚未显示有害效应,但过敏反应并非偶然,其发生率约为20%。此外,用造影剂作心血管扫描检查的病人,可检出淋巴细胞染色体损伤。关于造影剂的诱变性,目前还有争议。为探讨现代造影剂的细胞遗传学效应,本实验用各种造影剂的不同剂量对培养的淋巴细胞进行了诱发微核实验。实验主要有两部分:(1)取20名健康人外周血常规培养24小时后,加入不同剂量的各种造影剂,继续培养48小时。常规制片,染色后进行微核观察;(2)14名接受造影检查的病人于注入造影剂前取血1次,造影检查后1小时再取血1次,分
The sodium triiodobenzoate contrast agent has been widely used in radiological diagnosis. Current statistics show that about 10 million contrast agents are used annually in Europe and the United States. Although these contrast agents have not shown harmful effects, the allergic reaction is not accidental, and its incidence is about 20%. In addition, chromosomal lesions in lymphocytes can be detected in patients who have undergone a cardiovascular scan with a contrast agent. Contrast agent mutagenicity is currently still controversial. In order to investigate the cytogenetic effects of modern contrast agents, micronucleus experiments were performed on cultured lymphocytes using different doses of various contrast agents. There are two main parts of the experiment: (1) After 20 hours of routine culture of peripheral blood from 20 healthy people, add various doses of contrast media and continue to culture for 48 hours. Conventional production and micronucleus observation after staining; (2) 14 patients undergoing angiography were bled 1 time before injection of contrast medium and 1 hour after angiography.