婴儿秋季腹泻的病原,国内外有不少报道都证实,最重要的为轮状病毒(Rotavirus)。我省亦于1981年用电子显微镜直接观察法,从婴儿腹泻粪便中发现了轮状病毒。材料和方法材料采自郑州地区的秋季腹泻患儿,年龄在两岁以下,发病在三天以内由肛管采集粪样后。用 H—500电镜观察。新鲜样品经3500～4000转/分离心半小时,取上清液滴于附有 Formvar 膜的铜网上,经2%磷钨酸(PH6.4～6.8)负染色2～5分钟,然后电镜观 Infant autumn diarrhea pathogens, many reports at home and abroad have confirmed that the most important for the rotavirus (Rotavirus). In 1981, our province also used direct observation by electron microscopy to detect rotavirus from diarrhea in infants. Materials and Methods Materials were collected from children with autumnal diarrhea in Zhengzhou area under the age of two. After the onset of infection, fecal samples were collected from the anal canal within three days. H-500 electron microscopy. Fresh samples were centrifuged at 3500-4000 rpm for half an hour. The supernatant was dropped on a copper mesh with Formvar membrane and negatively stained with 2% phosphotungstic acid (pH 6.4 ~ 6.8) for 2 ~ 5 minutes. Then electron microscope