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自Blumberg 1964年发现乙型肝炎表面抗原(HBsAg)以来,检测HBsAg的新方法不断建立,特异性和敏感性不断提高。1974年Shikata等首先报道用地衣红染色显示石蜡切片中HBsAg以后,国内外均有资料用免疫酶标等方法证实了地衣红的特异性,其检出率也显著高于血清学阳性率(R-PH)。免疫酶染色的问世,其高敏感性和高特异性更显其优越性,组织中HBsAg的检出率更高于地衣红染色。我们对小肝癌癌周肝组织同时用地衣红染色和免疫酶标染色(PAP法)检测了HBsAg,并与血清学(R-PHA法)检测结果作了比较分析。
Since Blumberg discovered hepatitis B surface antigen (HBsAg) in 1964, new methods for detecting HBsAg have been established, with increasing specificity and sensitivity. Shikata et al. First reported the identification of lichen-red specificity in paraffin sections by using lichen-red staining in 1974, and the detection rate of lichen-red was also significantly higher than that of seroprevalence (R -PH). Advent of immune enzyme staining, its high sensitivity and high specificity even more its superiority, the detection rate of HBsAg in tissues is higher than lichen red staining. We detected HBsAg in liver tissues of small hepatocarcinoma with lichen red staining and immuno-enzyme-linked immunosorbent assay (PAP method) at the same time, and compared with the results of serological (R-PHA) assay.