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目的探讨长时间应用大剂量的左旋精氨酸对幼鼠学习记忆功能和神经系统可塑性的影响。方法分别给初断乳的大鼠灌胃大剂量的NO前体左旋精氨酸(L-arginine,L-Arg)[600 mg/(kg.d),L-Arg组]和同样剂量的蒸馏水(对照组),每组各10只,持续3个月。用Morris水迷宫检测大鼠的学习记忆功能,用免疫组化技术和HE染色检测大脑皮质和海马CA1、CA3、DG区的神经元型一氧化氮合酶(nNOS)和c-fos基因的表达以及神经细胞数目的变化。结果实验结果显示大鼠寻台潜伏期的时间和nNOS的表达在L-Arg组明显较对照组升高,但c-fos基因的表达和大脑皮质及海马CA1、CA3、DG区的神经元数目在L-Arg组明显较对照组降低(P<0.05或P<0.01)。结论长时间应用大剂量的NO前体L-Arg可抑制幼鼠的学习记忆功能和神经系统的可塑性。
Objective To investigate the effects of long-term administration of L-arginine on learning and memory function and neurological plasticity in young rats. Methods Rats with weaned milk were given intragastric administration of large doses of L-arginine (L-Arg) [600 mg / (kg · d), L-Arg] and the same dose of distilled water (Control group), each group of 10, for 3 months. Morris water maze was used to detect learning and memory in rats. Immunohistochemistry and HE staining were used to detect the expression of nNOS and c-fos genes in CA1, CA3 and DG of cerebral cortex and hippocampus As well as changes in the number of nerve cells. Results The results showed that the latency and the nNOS expression in rat L-Arg group were significantly higher than those in control group, but the expression of c-fos gene and the number of neurons in CA1, CA3 and DG of cerebral cortex and hippocampus were L-Arg group was significantly lower than the control group (P <0.05 or P <0.01). Conclusion Long-term application of large doses of NO precursor L-Arg can inhibit the learning and memory function and neurological plasticity in young rats.