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电活性生物膜形成、胞外电子传递机制以及环境效应研究已成为环境领域关注的热点.通过在碳毡上富集形成生物膜,采用电化学方法对生物膜的电活性进行了表征,对电活性生物膜直接介导Cu2+的还原转化进行了初探.利用扫描电子显微镜(SEM)、能谱分析仪(EDS)、X射线光电子能谱(XPS)对Cu元素的形貌、含量以及价态进行分析,结果表明电活性生物膜能够利用乙酸作为电子供体,Cu2+作为电子受体,将Cu2+还原转化成Cu或Cu+.同时,通过激光共聚焦显微镜(LSCM)观察Cu2+对微生物的影响,结果显示Cu2+对微生物有明显的毒害作用,所以电活性生物膜介导Cu2+的转化只能在较低的铜离子浓度下进行.本研究结果将为电活性生物膜应用于环境中铜离子的固定和回收提供理论依据.
Electro-active biofilm formation, extracellular electron transfer mechanism and environmental effects have become the focus of attention in the field of the environment. By biofilm enrichment on carbon felt, the electrochemical activity of biofilm was characterized by electrochemical method. The active biofilm directly mediates the reduction and transformation of Cu2 + .The morphologies, contents and valences of Cu elements were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray photoelectron spectroscopy The results showed that the electroactive biofilm could utilize acetic acid as the electron donor and Cu2 + as the electron acceptor to convert Cu2 + to Cu or Cu + .At the same time, the effect of Cu2 + on the microorganism was observed by laser confocal microscopy (LSCM) Cu2 + has a significant toxic effect on microorganisms, so electroactive biofilm-mediated Cu2 + conversion can only be carried out at a lower concentration of copper ions.The results of this study will be used for the immobilization and recovery of electroactive biofilm in the environment of copper ions Provide a theoretical basis.