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为了揭示X蛋白与HBV复制的关系,作者将肝内X蛋白的表达与肝和血清的各种HBV复制标记进行了比较。 59例HBsAg阳性患者,经组织学证实,18例为慢性迁延性肝炎(CPH),41例为慢性活动性肝炎(CAH)。患者肝标本的X蛋白用抗重组X蛋白的特异性抗体采取免疫组化法检测;前S_1和前S_2抗原用固相酶免疫法检测;HBV DNA用~(32)P标记的DNA
In order to reveal the relationship between X protein and HBV replication, the authors compared the intrahepatic X protein expression with various HBV replication markers in liver and serum. Of the 59 HBsAg positive patients, histologically confirmed 18 chronic persistent hepatitis (CPH) and 41 chronic active hepatitis (CAH). The X protein of patients’ liver specimen was detected by immunohistochemistry with the specific anti-X antibody; the former S 1 and the former S 2 antigens were detected by solid phase enzyme immunoassay; the HBV DNA was labeled with ~ (32) P DNA