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运用生精上皮周期理论,以定量组织学方法,结合采用电镜组化技术,观察了~(60)Coγ射线全身照射大鼠后睾丸生精上皮、曲细精管界膜和界膜内碱性磷酸酶的改变。探讨了~(60)Coγ射线对生精上皮的损伤特点,曲细精管界膜内碱性磷酸酶的辐射敏感性,以及界膜和界膜内碱性磷酸酶与生精上皮在辐射损伤、修复中的相互关系。结果显示,分化型精原细胞对辐射最敏感;4Gyγ射线全身照射后,大鼠生精上皮仍可恢复,幸存的耐辐射精原干细胞是修复上皮的唯一来源;支持细胞超微结构随照射后生精细胞数量的减少而改变;照射后界膜明显增厚、分层、皱折;界膜内碱性磷酸酶的辐射耐受性较高,受照后12天碱性磷酸酶反应产物明显减少,其恢复早于生精上皮的修复。
Using the theory of germinal epithelium cycle, quantitative histological method combined with electron microscopy was used to observe the effects of ~ (60) Coγ-ray irradiation on the spermatogenic epithelium, Changes in phosphatase. The damage characteristics of ~ (60) Coγ-ray on the seminiferous epithelium, the radiosensitivity of alkaline phosphatase in the seminiferous tubule and the effects of alkaline phosphatase and spermatogenic epithelium on the radioactive injury , Repair the relationship between. The results showed that the differentiation of spermatogonia is the most sensitive to radiation; 4Gy γ-ray after irradiation, the rat spermatogenic epithelium can still be restored, the survival of the radiation-resistant spermatogonial stem cells repair the only source of epithelium; support cell ultrastructure with irradiation The number of sperm cells decreased and changed; after irradiation, the boundary membrane was thickened, stratified and wrinkled; the radioactivity of alkaline phosphatase in the boundary membrane was higher, and the products of alkaline phosphatase reaction significantly decreased 12 days after irradiation , Which restored earlier than the repair of the seminiferous epithelium.