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采用温室盆栽方法,研究了持续干旱及复水处理后,接种褐环乳牛肝菌7、牛肝菌1、鸡油菌、彩色豆马勃和土生空团菌的马尾松苗木生理变化,并对菌根化苗木进行抗旱性评价.结果表明:在持续干旱条件下,马尾松苗木的丙二醛和相对质膜透性随之增加,但菌根化苗木的丙二醛和相对质膜透性均显著低于未接种苗木(对照);复水后,菌根化苗木中丙二醛和质膜透性较对照迅速降低.在持续干旱胁迫前21d,马尾松苗木超氧阴离子自由基产生速率增加,同时也诱导了菌根化苗木中过氧化物歧化酶、过氧化物酶和硝酸还原酶活性显著增加.随着胁迫时间的延长,苗木复水后的恢复能力各异.在胁迫14d复水后,苗木过氧化物歧化酶、过氧化物酶和硝酸还原酶的活性均得以恢复.菌根化苗木抗旱性的强弱为牛肝菌7>牛肝菌1>鸡油菌>土生空团菌>彩色豆马勃.过氧化物歧化酶和丙二醛与马尾松菌根化苗木抗旱性关联度较大,可以作为抗旱鉴定的主要指标.
A greenhouse pot experiment was conducted to study the physiological changes of Pinus massoniana seedlings inoculated with A. bivalviae 7, porcini 1, chanterelles, color beans and Boletus sibiricus after continuous drought and rewatering Mycorrhizal seedlings were evaluated for drought resistance.The results showed that the MDA and relative plasma membrane permeability of P. massoniana seedlings increased under continuous drought conditions, but the malondialdehyde and relative plasma membrane permeability of mycorrhized seedlings Were significantly lower than those of the uninoculated seedlings (control). After rewatering, the malondialdehyde and plasma membrane permeability of mycorrhized seedlings decreased rapidly compared with the control.At 21 d before the continuous drought stress, the production rate of superoxide anion radical But also increased the activities of superoxide dismutase, peroxidase and nitrate reductase in mycorrhizal seedlings at the same time.With the prolongation of stress time, Water, seedlings of superoxide dismutase, peroxidase and nitrate reductase activity were restored.The mycorrhizal seedlings drought resistance strength Boletus 7> Boletus 1> chanterelles> soil empty The bacteria> colored bean Bo. Superoxide dismutase and malondialdehyde with horse The results showed that there was a significant correlation between the drought resistance and the mycorrhizal fungi seedlings, which could be used as the main index of drought resistance identification.