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目的了解前列腺素E2在尿结石形成中的作用以及α-亚麻酸对草酸钙结石鼠前列腺素E2、肾草酸钙结晶形成的抑制作用。方法60只雄性Wistar成年大鼠随机分成4组,分别喂饲苏子油、葵花籽油4周后,使用诱石剂1%乙二醇(EG)加1%氯化氨喂饮大鼠,同时继续以苏子油和葵花籽油灌胃,3周后检测各组大鼠肾功能,24h血尿生化指标,肾草酸钙结晶情况及各组大鼠尿前列腺素E2水平。结果苏子油组肾组织水肿较轻,肾内草酸钙结晶数及肾钙含量明显低于成石组(P<0·01),24h尿钙排泄,血尿素氮,肌酐浓度显著低于成石组(P<0·05),尿肌酐排泄增加(P<0·05)。葵花籽油组仅血尿肌酐较成石组明显改善(P<0·05),其他指标与成石组无显著差异(P>0·05)。24h尿前腺素E2水平苏子油组、对照组显著低于成石组(P<0·01、P<0·05)。葵花籽油组与成石组比较无显著性差异(P>0·05)。成石组、苏子油组、葵花籽油组前列腺素E2水平与尿总钙含量成正相关性。结论前列腺素E2可能参与并促进了尿结石的形成,α-亚麻酸可能通过抑制肾前列腺素E2的产生而抑制尿结石形成。
Objective To investigate the role of prostaglandin E2 in the formation of urinary calculus and the inhibitory effect of α-linolenic acid on the formation of oxaliplatin-prostaglandin E2 and renal calcium oxalate crystals. Methods Sixty adult male Wistar rats were randomly divided into 4 groups. Rats were fed with sunflower seed oil and sunflower seed oil for 4 weeks, respectively. Rats were fed with 1% ethylene glycol (EG) and 1% ammonium chloride. At the same time continue to sufu oil and sunflower oil gavage, 3 weeks after the detection of renal function in rats, 24h urine biochemical indicators, renal calcium oxalate crystallization and urinary prostaglandin E2 levels in each group. Results The edema of renal tissue in Suzi oil group was lighter, the number of calcium oxalate in kidney and the content of renal calcium in kidney were significantly lower than that in Chengshi group (P <0.01), excretion of urinary calcium in 24h, blood urea nitrogen and creatinine concentration were significantly lower than those in Cheng Stone group (P <0 · 05), urinary creatinine excretion increased (P <0 · 05). In the sunflower oil group, only hematuria and creatinine were significantly improved (P <0.05), but no significant difference was found between the other indexes and the stone forming group (P> 0.05). The level of 24h urinary prostaglandin E2 was significantly lower in the Su-oil group than in the control group (P <0.01 or P <0.05). No significant difference was found between sunflower oil group and Chengshi group (P> 0.05). There was a positive correlation between prostaglandin E2 level and total urinary calcium in Chengshu group, Suzi group and sunflower oil group. Conclusions Prostaglandin E2 may participate in and promote the formation of urolithiasis. Α-linolenic acid may inhibit the formation of urinary stones by inhibiting the production of prostaglandin E2.