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目的：分离脐血CD细胞，以了解其造血增殖活性。方法：应用免疫磁珠法（Isolex50系统）分离脐血CD细胞，并对其实验方法进行了改进。结果：常规方法CD纯度较低，经过改进，脐血CD细胞由分离前的（1．39±0．76）％增加至（59．46±17．35）％，CD细胞富集倍数为（62．31±55．19）倍。分离后组分CFU－GM、BFU－E、CFU－Mix较分离前分别增加了（41．05±15．77）、（13．14±8.66）及（34．80±19．58）倍，而阴性组分所形成的集落显著减少。结论：免疫磁珠法可以有效地分离CD细胞，造血祖细胞集落大多来源于CD细胞。
OBJECTIVE: To isolate cord blood CD cells to understand their hematopoietic and proliferative activity. Methods: Cord blood CD cells were isolated by immunomagnetic beads method (Isolex50 system), and the experimental method was improved. Results: The CD purity of conventional method was lower and improved. The percentage of CD cells in cord blood increased from (1.39 ± 0.76)% to (59.46 ± 17.35)% before isolation and ( 62.31 ± 55.19) times. The fraction of CFU-GM, BFU-E and CFU-Mix increased by (41.05 ± 15.77), (13.14 ± 8.66) and (34.80 ± 19.58) Negative components formed colonies significantly reduced. Conclusion: Immunomagnetic beads method can effectively separate CD cells. Most of hematopoietic progenitor colony originated from CD cells.