Brain injury due to acute organophosphate poisoning Magnetic resonance imaging manifestation and pat

来源 :Neural Regeneration Research | 被引量 : 0次 | 上传用户:William_hui
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
BACKGROUND: Acute organophosphate poisoning can cause injuries of multiple visceras; especially, central nervous system injury can increase risk factors of patients with severe acute organophosphate poisoning. An application of modern image may increase diagnostic rate of brain injury in an earlier period and provide evidences for clinical treatment. OBJECTIVE: To reveal imaging manifestations, pathological characteristics and multi-ways injured mechanism of brain injury due to acute organophosphate poisoning. DESIGN: Contrast observational study. SETTING: Department of Medical Image, the Second Hospital of Hebei Medical University. MATERIALS: The experiment was carried out in the Department of Nerve Molecule Imaging Medicine and Laboratory of Neurology, the Second Hospital of Hebei Medical University from August 2003 to February 2004. A total of 30 healthy cats weighing 2.8–3.5 g and of both genders were selected from Animal Experimental Center of Hebei Medical University. METHODS: Thirty healthy cats were randomly divided into control group (n =5) and intoxication group (n =25). Cats in the control group were subcutaneously injected with 0.3 mL/kg saline at four points; while, cats in the intoxication group were subcutaneously injected with 400 g/L 0.3 mL/kg O,O-dimethyl-S-(methoxycarbonylmethyl) thiophosphate at four points. Two minutes after intoxication, cats received muscular injection with 0.5 mg/kg atropine sulfate, and then, brain tissues were collected from parietal lobe, basal ganglia, hippocampus, cerebellum and brain stem were observed at 3, 6, 24 hours, 3 and 7 days after intoxication respectively under optic microscope and electron microscope and expressions of acetylcholinesterase (AChE), choline acetyltransferase (ChAT), glial fibrillary acidic protein (GFAP), glutamic acid (Glu) and γ-amino butyric acid after immunohistochemical staining. MAIN OUTCOME MEASURES: Results of MRI examinations; histological changes under optic microscope and electron microscope; expressions of AChE, ChAT, GFAP, Glu and γ-amino butyric acid after immunohistochemical staining. RESULTS: All 30 healthy cats were involved in the final analysis. ① Imaging and pathological observation: Image manifestations of brain injury induced by acute organophosphate poisoning showed as cerebral edema and symmetry signal abnormality of bilateral basal ganglia; while, pathological manifestations also showed as cerebral edema. ② Observation of immunohistochemical staining: As compared with the control group, after organophosphate poisoning, area of AChE immune-positive cells was decreased obviously (P < 0.01), but area of ChAT immune-positive cells was not changed (P > 0.05); in addition, positive cells of GFAP were increased remarkably (P < 0.01), positive cells of γ-amino butyric acid in cerebral cortex were increased obviously (P < 0.05), but numbers of positive cells of Glu were not changed (P < 0.05). CONCLUSION: Multi-ways injured mechanism invovled in acute organophosphate poisoning. An application of modern image can increase diagnostic rate of brain injury in an earlier period and provide evidences for clinical treatment. BACKGROUND: Acute organophosphate poisoning can cause injuries of multiple visceras; especially, central nervous system injury can increase risk factors of patients with severe acute organophosphate poisoning. An application of modern image may increase diagnostic rate of brain injury in earlier period and provide evidences for clinical treatment. OBJECTIVE: To reveal imaging manifestations, pathological characteristics and multi-ways injured mechanism of brain injury due to acute organophosphate poisoning. DESIGN: Contrast observational study. SETTING: Department of Medical Image, the Second Hospital of Hebei Medical University. MATERIALS: The experiment was carried out in the Department of Nerve Molecule Imaging Medicine and Laboratory of Neurology, the Second Hospital of Hebei Medical University from August 2003 to February 2004. A total of 30 healthy cats weighing 2.8-3.5 g and of both genders were selected from Animal Experimental Center of Hebei Medical University. METHODS: Thirty healthy cats were randomly divided into control group (n = 5) and intoxication group (n = 25). Cats in the control group were subcutaneously injected with 0.3 mL / kg saline at four points; while, cats in the intoxication group were subcutaneously injected with 400 g / L 0.3 mL / kg O, O-dimethyl-S- (methoxycarbonylmethyl) thiophosphate at four points. Two minutes after intoxication, cats received muscular injection with 0.5 mg / kg atropine sulfate, and then, from parietal lobe, basal ganglia, hippocampus, cerebellum and brain stem were observed at 3, 6, 24 hours, 3 and 7 days after intoxication respectively under optic microscope and electron microscope and expressions of acetylcholinesterase (AChE), choline acetyltransferase (ChAT) glial fibrillary acidic protein (GFAP), glutamic acid (Glu) and γ-amino butyric acid after immunohistochemical staining. MAIN OUTCOME MEASURES: Results of MRI examinations; histological changes under optic microscope and electron miRESULTS: All 30 healthy cats were involved in the final analysis. ① Imaging and pathological observation: Image manifestations of brain injury induced by acute organophosphate poisoning showed as cerebral edema and symmetry signal abnormality of bilateral basal ganglia; while, pathological manifestations also showed cerebral edema. ② Observation of immunohistochemical staining: As compared with the control group, after organophosphate poisoning, area of ​​AChE immune-positive cells was decreased obviously (P <0.01), but area of ​​ChAT immune-positive cells was not changed (P> 0.05); in addition, positive cells of GFAP were increased remarkably (P <0.01) were increased obviously (P <0.05), but numbers of positive cells of Glu were not changed (P <0.05). CONCLUSION: Multi-ways of injured mechanism invovled in acute o An application of modern image can increase the diagnostic rate of brain injury in an earlier period and provide evidences for clinical treatment.
其他文献
目的 探讨循环免疫复合物 (CIC)在乙肝发病机制上的作用。方法 应用聚乙二醇 (PEG)沉淀试验对 1 2 0例乙肝病人和 1 2 0例正常人群进行了循环免疫复合物检测。结果 乙肝病
世界进入了隐身空军的时代,我们不能不了解隐身时代的战斗机!1989年12月20日,两架 F-117隐身战斗机飞临巴拿马里奥阿托军营上空,各投下一枚激光制导炸弹。随着“轰——轰—
作文训练是综合性、应用性很强的实践活动,因此,它的任务也就必然具有广泛性和针对性;由于作文训练是一个科学的系统过程,注重的是整体素质的渐次提高。概括说来,作文训练所
近年来,高效抗逆转录病毒疗法(HAART)的广泛应用,明显降低了HIV感染者的死亡危险性(估计较HAART前下降85%以上)。但与年龄配对的非HIV感染者相比,其病死率仍明显较高。为了评
去年,我参加了松原市小学数学同课异构课堂教学研讨会,受益匪浅。尤其是“三角形的面积”这一教学内容,执教者在讲授时把学习的主动权还给学生,教师只做必要的指导。让学生动
女性,54岁,工人,已婚,汉族.因背腰部肌肉疼痛5 d于2005年6月18日入院.患者10余天前咽喉疼痛,应用静脉点滴青霉素,口服罗红霉素等治疗后咽痛减轻.5 d前出现背腰部肌肉疼痛,以脊柱两侧为著.疼痛呈持续性、刀刺感,难以忍受,曾在门诊按"肌纤维组织炎"而服用双氯芬酸钠治疗无效.16日夜间服用安乃近每次2片,共4次,疼痛仍未止。
目的 探讨土源性线虫病高度流行区经济、有效、易行的化疗策略。 方法 在 3个自然村随机采用 3种全民化疗方案 ,即首次化疗后 ,每隔 3个月、6个月和 12个月重复化疗 1次 ,
目的:为了了解西安地区急性GBV-C/HGV感染者的分子流行病学及临床特征。 方法:连续收集急性病毒性肝炎病例458例,诊断符合上海会议标准。经EHSA检测的67例非甲-戊型肝炎患者
2010年明中旬.江苏省通信管理局组织专项检查队伍,对南京、常州、昆山、常熟、江阴等地的相关互联网企业的网站备案真实性核验工作以及增值电信业务市场情况进行专项检查。在检
数学知识源于生活而最终服务于生活。如果数学教中教师只为了教而教,把知识生硬地教给学生,而对于学生来说,他们就会越来越感到数学是枯燥的,是冷冰冰的,学习数学只是为了完