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通过温室水培试验,研究1.0×10-4mol/LZn2+胁迫及解除胁迫对番茄(Lycopersicom esculentum Mill.)幼苗根系中抗氧化系统的动态变化.结果表明,胁迫条件下,番茄根系丙二醛(MDA)和蛋白质含量增加;超氧化物歧化酶(SOD,EC1.15.1.1),过氧化氢酶(CAT,EC1.11.1.6)和谷胱甘肽过氧化物酶(GPX,EC1.11.1.9)活性上升,而过氧化物酶(POD,EC1.11.1.7)活性下降;AsA-GSH循环代谢受影响;抗坏血酸过氧化酶(APX,EC1.11.1.11)活性在胁迫d1、d3上升,随后下降,而谷胱甘肽还原酶(GR,EC1.6.4.2)活性在胁迫1~5d时下降,随后上升;抗坏血酸(AsA)和谷胱甘肽(GSH)含量上升.解除胁迫后,根系中MDA和蛋白质含量随时间延长逐渐减少,接近对照;POD和APX活性增加,而SOD、CAT和GR活性及GSH含量先升高,随后减少;GPX活性和AsA含量却降低;恢复后根系中抗氧化物水平仍然高于对照.依据实验结果,文中讨论了胁迫及恢复过程中植物AsA-GSH循环代谢的作用.图3参16
The dynamic changes of antioxidant system in roots of tomato (Lycopersicum esculentum Mill.) Seedlings under 1.0 × 10-4 mol / L Zn2 + stress and stress release were studied by hydroponics experiment in greenhouse. The results showed that under drought stress, malondialdehyde (MDA) ) And protein content increased; superoxide dismutase (SOD, EC1.15.1.1), catalase (CAT, EC1.11.1.6) and glutathione peroxidase (GPX, EC1.11.1. 9), while the activity of peroxidase (POD, EC1.11.1.7) decreased; the metabolism of AsA-GSH was affected; the activity of ascorbate peroxidase (APX, EC1.11.1.11) , And then decreased, while the activity of glutathione reductase (GR, EC1.6.4.2) decreased from 1 to 5 days of stress and then increased, and the content of asA and GSH increased , MDA content and protein content in root decreased gradually with time, approaching the control; POD and APX activity increased, while SOD, CAT and GR activity and GSH content first increased, then decreased; GPX activity and AsA content decreased; The level of antioxidants is still higher than that of the control.According to the experimental results, the role of plant AsA-GSH cycle metabolism in stress and recovery process is discussed. 3 Reference 16