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目的 :探讨慢性乙型肝炎患者血清、肝组织、外周血单个核细胞 (PBMC)中病毒载量的相互关系 ;初步明确PBMC病毒量与肝细胞损伤之间的关系。方法 :5 0例慢性乙型肝炎患者均接受肝活检。一部分肝穿标本进行常规病理检查 ,另一部分用于HBVDNA定量检测。同时分离患者PBMC ,用于病毒载量分析。病毒定量采用荧光定量PCR技术。同时收集患者的生化检查资料。通过SAS 8 .0软件对相关数据进行统计分析。结果 :PBMC中HBVDNA载量与肝组织HBVDNA载量呈中度正相关 (r =0 . 5 3 85 5 ,P <0 . 0 0 0 1) ;与血清病毒载量也呈正相关 (r =0 . 483 16,P =0 . 0 0 0 6) ;与血清转氨酶、肝组织炎症活动度、纤维化分期无明显相关性。以PBMC病毒载量作为因变量 ,进行逐步回归分析。得到逐步回归方程 :Y =3 713 0 1+ 0 . 16946X (Y :PBMC病毒载量 ;X :肝组织病毒载量 )。结论 :PBMC中病毒载量受肝组织病毒量的显著影响 ,一定程度上提示PBMC中可能存在复制型的HBV。
Objective: To investigate the relationship between the viral load in serum, liver tissue and peripheral blood mononuclear cells (PBMC) in patients with chronic hepatitis B and to clarify the relationship between the amount of PBMC virus and hepatocellular injury. Methods: Fifty patients with chronic hepatitis B underwent liver biopsy. A portion of the liver specimens for routine pathological examination, the other part for quantitative detection of HBVDNA. Patient PBMCs were also isolated for viral load analysis. Quantitative PCR using quantitative PCR. At the same time to collect biochemical examination of patients information. Through SAS 8 .0 software for statistical analysis of the relevant data. Results: There was a moderate positive correlation between HBVDNA load and HBVDNA load in PBMC (r = 0. 5 3 85 5, P 0. 0 01), and positive correlation with serum viral load .483 16, P = 0. 0 0 0 6); and serum aminotransferase, liver inflammation activity, fibrosis staging no significant correlation. PBMC viral load as a dependent variable, stepwise regression analysis. A stepwise regression equation was obtained: Y = 3 713 0 1 + 0.16946X (Y: PBMC viral load; X: liver tissue viral load). CONCLUSION: The viral load in PBMC is significantly affected by the amount of virus in liver tissue, suggesting to some extent that there may be replicative HBV in PBMC.