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目的:探讨桑黄菌液体发酵的适宜培养基,为进一步研究其液体发酵工艺及提取有效成分做准备。方法:首先用单因素单指标法筛选,再用正交试验双指标法筛选。结果:桑黄菌生长所需最适C/N比为24;最适的碳源为黄豆粉和玉米粉,适宜的含量分别为4%和3%;最适的氮源为小麦麸粉,适宜的含量为0.5%;对菌丝生长发育影响显著的物质有FeSO4、ZnSO4、K2SO4、CaSO4、Na2HPO4、VB1等。结论:桑黄菌液体发酵适宜的培养基配方(g/L):黄豆粉40、玉米粉30、小麦麸粉5、酵母浸出汁4、CaSO410、K2SO41、Na2HPO41.5、ZnSO4.7H2O0.1、Fe-SO4.7H2O0.5、VB10.1。
OBJECTIVE: To investigate the suitable culture medium for liquid fermentation of Phellinus igniarius, in order to further study its liquid fermentation process and extract the active ingredients. Methods: The first single factor single factor screening, and then orthogonal test double index screening. Results: The optimum C / N ratio for the growth of Phellinus igniarius was 24. The optimum carbon sources were soybean flour and corn flour, the suitable contents were 4% and 3% respectively. The optimum nitrogen source was wheat flour, The suitable content is 0.5%. The substances that have significant effect on mycelium growth and development are FeSO4, ZnSO4, K2SO4, CaSO4, Na2HPO4 and VB1. CONCLUSION: The optimum medium for liquid fermentation of Phellinus igniarius (g / L) is as follows: soybean flour 40, corn flour 30, wheat bran flour 5, yeast extract 4, CaSO410, K2SO41, Na2HPO41.5, ZnSO4.7H2O0.1, Fe-SO4.7H2O0.5, VB10.1.