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用0~3.0mg/L浓度GeO2对水稻种子浸种处理之后,在木村B培养液中培养,并分别以6个浓度继续添加GeO2溶液.另用不同浓度的GeO2和SiO2混合溶液培养水稻,测定其营养生长期的整株鲜重、根鲜重、地上部高、叶绿素含量、根系活力以及叶过氧化氢酶(CAT)、叶多酚氧化酶(PPO)、根过氧化物酶(根PO)和叶(PO).实验表明,随着培养时间延长,上述生理指标值有所变化,其中前6项正常生理指标值,随着GeO2浓度提高而减少,后3项衰老指标则随着GeO2浓度提高而有增加趋势,表明3种酶活性的提高以缓解GeO2对水稻的毒害作用.各生理指标与GeO2浓度之间呈明显相关关系.同时,还发现了一定浓度的GeO2对水稻生长有抑制和毒害作用,而较高浓度的SiO2可适度缓解该抑制作用.
After soaking rice seeds with 0 ~ 3.0mg / L GeO2, the seedlings were cultivated in Kimura B medium, and GeO2 solution was further added at 6 concentrations respectively. In addition, different concentrations of GeO2 and SiO2 were used to culture rice. The fresh weight, root fresh weight, shoot height, chlorophyll content, root activity and leaf catalase (CAT), leaf polyphenol Oxidase (PPO), root peroxidase (root PO) and leaf (PO). The experimental results showed that the above physiological indices changed with the prolongation of culture time. Among them, the first 6 physiological indexes decreased with the increase of GeO2 concentration, while the latter 3 increased with the increase of GeO2 concentration. The results showed that the activity of three kinds of enzymes increased to alleviate the toxic effect of GeO2 on rice. The physiological index and GeO2 concentration showed a significant correlation between. At the same time, it was also found that certain concentrations of GeO2 inhibited and poisoned the growth of rice, while the higher concentration of SiO2 could moderately alleviate the inhibition.