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迄今，骨髓辐射损伤后造血细胞凋亡的病理研究很少，其定量变化规律更未见报道。本文采用６０Ｃｏγ射线照射ＬＡＣＡ小鼠，于照射后６小时、１天及３天分批活杀，将右股骨骨髓经３％戊二醛及１％锇酸固定，制作半薄切片、超薄切片，并用ＣａｍｂｒｉｄｇｅＱｕａｎｔｉｍｅｔ９７０型图像分析仪，定量研究了辐射诱导的骨髓造血细胞凋亡的形态特征及其与照射剂量之间的关系。结果表明：对照组动物骨髓仅有少数造血细胞凋亡，而照射后６小时各剂量组动物骨髓内发生凋亡的造血细胞均明显增多，病理特点为：凋亡早期见核染色质浓缩、边移，呈半月形、环形或不规则形；中期见大小不等，形状不一的染色质团块周围有核膜包绕，核碎片形成；晚期见凋亡小体形成，可游离于细胞间或被邻近细胞吞噬。定量分析示各照射组动物凋亡的骨髓造血细胞的数密度和面密度均明显增加，与对照组比，其差异显著（Ｐ＜００１）。且剂量越大，凋亡细胞的数密度和面密度越大。最后，本文作者根据实验结果指出：凋亡是辐射介导的骨髓造血细胞死亡的主要死亡方式。本研究为急性放射病的诊治提供直接依据，亦可能对其他原因引起的细胞凋亡的研究具有参考意义。
So far, pathological studies on the apoptosis of hematopoietic cells after bone marrow irradiation have been scarce, and the quantitative changes have not been reported yet. In this paper, LACA mice were irradiated with 60Coγ ray and killed in 6 hours, 1 day and 3 days after irradiation. The right femur bone marrow was fixed with 3% glutaraldehyde and 1% osmium tetroxide to make semi-thin section and ultra-thin section , And using CambridgeQuantimet970 image analyzer quantitative analysis of radiation-induced morphological characteristics of bone marrow hematopoietic cells apoptosis and irradiation dose relationship. The results showed that only a few apoptotic hematopoietic cells were found in the bone marrow of the control group, while the apoptotic hematopoietic cells in the bone marrow of each dose group were significantly increased at 6 hours after irradiation. The pathological features were as follows: nuclear chromatin condensation Move, showed a semilunar, ring or irregular shape; see the medium-term size, chromatin around the shape of the nuclear membrane surrounded by nuclear debris formation; late see apoptotic bodies formed, can be separated in the intercellular or Phagocytosis by neighboring cells. Quantitative analysis showed that the number density and the areal density of bone marrow hematopoietic cells in the irradiated groups were significantly increased compared with the control group (P <001). The larger the dose, the greater the number density and area density of apoptotic cells. Finally, according to the experimental results, the author points out that apoptosis is the main way of death mediated by radiation-induced bone marrow hematopoietic cells. This study provides a direct basis for the diagnosis and treatment of acute radiation sickness, may also be of reference value for the study of apoptosis caused by other causes.