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目的 探讨表皮生长因子 (EGF)及其受体 (EGFR)在甲状腺增殖性疾患中的表达及其作用。方法 应用免疫组化ABC染色方法观察 70例甲状腺组织切片EGF及EGFR的表达。结果(1)EGF在正常、肿瘤及非肿瘤组织中几乎均无表达。 (2 )乳头状、滤泡型甲状腺癌及其混合癌EGFR阳性表达高于非癌疾患及正常组织 (P <0 .0 5 ) ,阳性表达程度以强阳性为主。 (3)在正常组织、良性腺瘤、结节性甲状腺肿及桥本病 ,弱或中度的EGFR阳性表达率各组间差异无显著性。正常组织阳性表达率虽高达 83.3 % ,但 2 / 3表达为弱阳性。 (4 )乳头状甲状腺癌以细胞浆表达EGFR占优势 ,滤泡型及混合型甲状腺癌主要为混合着色 ,但与非癌混合着色不同的是多数以胞浆表达占优势 ;良性疾患以混合染色为主 ,但结节性甲状腺肿以膜着色居多。正常组织为浆、膜混合着色。结论 (1)对EGFR强阳性表达尤其细胞浆为主者应高度疑及甲状腺癌。 (2 )各甲状腺良性疾患均有不同程度EGFR表达 ,虽无统计学意义上的差别 ,却可说明EGFR对于良性肿瘤及非肿瘤增殖性疾患的生成均有不应忽视的作用。
Objective To investigate the expression and its role of epidermal growth factor (EGF) and its receptor (EGFR) in thyroid proliferative disorders. Methods Immunohistochemical ABC staining method was used to observe the expression of EGF and EGFR in 70 cases of thyroid tissue. Results (1) EGF was almost not expressed in normal, tumor and non-tumor tissues. (2) The positive expression of EGFR in papillary and follicular thyroid carcinoma and its mixed carcinomas was higher than that in non-cancerous and normal tissues (P <0.05), and the positive expression was strongly positive. (3) In normal tissues, benign adenomas, nodular goiter and Hashimoto’s disease, there was no significant difference between the groups in the expression of weak or moderate EGFR. Although the positive expression rate of normal tissue was as high as 83.3%, 2/3 expression was weakly positive. (4) The expression of EGFR in cytoplasm of papillary thyroid carcinoma predominates. Follicular and mixed thyroid carcinoma are mainly mixed coloration, but different from non-cancerous mixed coloration, most of them are expressed in cytoplasm. Benign disorders are mixed staining Mainly nodular goiter to the majority of membrane staining. Normal tissue is pulp, film mixed coloring. Conclusions (1) Strong positive expression of EGFR, especially cytoplasm should be highly suspected thyroid cancer. (2) The thyroid benign diseases have different degrees of EGFR expression, although there is no statistically significant difference, but it can explain the EGFR for benign tumors and non-tumor proliferative disorders are generated should not be ignored.