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目的：建立Dot—ELISA法检测荨麻疹患者血清中特异性IgG抗体。方法：采用斑点一酶联免疫吸附试验测定人血清中特异性IgG抗体，并进行特异性，敏感性试验。结果：慢性荨麻疹的抗过敏原的特异性IgG抗体明显高于急性荨麻疹的同类抗体，而且特异性IgG抗体的出现与荨麻疹的严重程度呈反比。结论。该法具有成本低，操作简便，观察结果直接等优点，可进行临床大批量标本的测定。
Objective: To establish a Dot-ELISA for the detection of serum IgG in urticaria patients. Methods: The specific IgG antibodies in human serum were determined by dot-ELISA, and the specificity and sensitivity were tested. Results: Chronic urticaria anti-allergen-specific IgG antibodies were significantly higher than those of acute urticaria and the presence of specific IgG antibodies was inversely proportional to the severity of urticaria. in conclusion. The method has the advantages of low cost, simple operation, direct observation results and the like, and can be used for the determination of clinical large-volume specimens.