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为准确测定血浆中尼莫地平浓度，本文建立了离子抑制性反相高效液相色谱法。血浆样品碱化后以乙醚－氯仿混合溶剂提取，提取液挥干，甲醇溶解后进样分析。采用μ－ＢｏｕｄａｐａｋＣ＿（１８）径向加压柱系统，甲醇－磷酸盐缓冲液为流动相，紫外检测波长为２３６ｎｍ。选择尼群地平为内标，峰面积定量。在１０～２００ｎｇ／ｍｌ内线性关系良好（ｒ＝０．９９８７），方法稳定可靠。
In order to accurately determine the concentration of nimodipine in plasma, ion-suppressed reversed-phase high performance liquid chromatography was established in this paper. The plasma samples were alkalified and extracted with ether-chloroform mixed solvent. The extract was evaporated to dryness and dissolved in methanol for injection analysis. The μ-BoudapakC_ (18) radial column system was used. The mobile phase consisted of methanol-phosphate buffer and the UV detection wavelength was 236 nm. Select nitrendipine as internal standard, peak area quantitative. In 10 ~ 200ng / ml within a good linear relationship (r = 0.9987), the method is stable and reliable.