论文部分内容阅读
目的建立HPLC法测定绿衣枳壳药材中葡萄内酯含量的方法。方法采用Diamonsil C18(150mm×4.6 mm,5 m),流动相:乙腈-水(80∶20),流速1.0ml·min~(-1),检测波长324nm,柱温25℃。结果葡萄内酯在0.088 96~0.533 76μg(r=0.9999)的进样量范围内呈良好的线性关系;其平均回收率(n=6)为98%,RSD为0.5%。结论该文法准确、简便,可用于绿衣枳壳药材中葡萄内酯的含量测定。
OBJECTIVE To establish a HPLC method for the determination of grape lactone in Fructus aurantii. Methods Diamonsil C18 (150 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile and water (80:20). The flow rate was 1.0 ml · min -1. The detection wavelength was set at 324 nm and the column temperature was 25 ℃. Results The results showed that there was a good linear relationship between the content of the lactone and the injection volume of 0.08896-0.53376μg (r = 0.9999). The average recoveries (n = 6) were 98% and the RSD was 0.5%. Conclusion The grammar is accurate and simple and can be used for the determination of the content of grape lactone in Fructus aurantii.