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分裂原反应性是检测免疫活性细胞功能的重要手段,已为国内外许多实验室采用。但目前国内临床实验室尚以形态学检测为主,具有主观误差较大的缺点;国外则多用~3H·TdR掺入法。此法需采用分离的人外周血淋巴细胞(PBL)作为反应细胞,手续繁杂,阻碍其在临床实践中的广泛应用。
Mitogen reactivity is an important means of detecting the function of immunocompetent cells and has been adopted by many laboratories both at home and abroad. However, at present, the clinical laboratory still takes the morphological test as the main method, which has the disadvantage of large subjective error. In the foreign countries, the 3H-TdR incorporation method is mostly used. This method requires the use of isolated human peripheral blood lymphocytes (PBL) as a reactive cell, complicated procedures, hindering its widespread use in clinical practice.