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目的探讨二氢杨梅素对60 Co-γ射线所致小鼠氧化损伤的保护作用。方法健康雄性昆明小鼠按体质量随机分为正常对照组、辐射对照组和二氢杨梅素(低、中、高)剂量组,各组均采用灌胃给药方式。辐射对照组和二氢杨梅素组小鼠全身一次性进行60 Co-γ照射,总辐射剂量为3.25Gy,各组继续给药7d后检测小鼠外周全血血清中过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶活性改变和丙二醛含量的变化。结果与辐射对照组比较二氢杨梅素高剂量组小鼠血清中超氧化物歧化酶、谷胱甘肽过氧化物酶活性显著升高(P<0.05),丙二醛含量显著降低(P<0.05);二氢杨梅素中、高剂量组小鼠血清中过氧化氢酶活性显著升高(P<0.05),与正常对照组比较各项指标无统计学差异。结论二氢杨梅素对60 Co-γ辐射小鼠引起的氧化应激损伤具有保护作用,其机制可能和抗氧化作用有关。
Objective To investigate the protective effect of dihydromyricetin on oxidative damage induced by 60 Co-γ-ray in mice. Methods Healthy male Kunming mice were randomly divided into normal control group, radiation control group and dihydromyricetin (low, medium and high) dose groups according to the body weight. Each group was given intragastric administration. Radiation control group and dihydromyricetin group mice were treated with 60 Co-γ radiation at a time, the total radiation dose was 3.25Gy. The mice in each group were continuously administered for 7 days and then the catalase, superoxide Changes in the activities of dismutase, glutathione peroxidase and malondialdehyde. Results Compared with the radiation control group, the superoxide dismutase and glutathione peroxidase activities were significantly increased (P <0.05) and the content of malondialdehyde was significantly decreased ). The catalase activity in serum of high and middle dose of dihydromyricetin was significantly increased (P <0.05). There was no significant difference between the two groups in the indexes of normal control. Conclusions Dihydromyricetin has a protective effect on oxidative stress induced by 60 Co-γ radiation in mice, and its mechanism may be related to anti-oxidative effects.