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棉花是一种典型的喜钾经济作物,其产量和品质形成与钾含量密切相关。克隆获得棉花高亲和性钾转运体基因GhHAK1框,系统进化树表明该GhHAK1基因属于第Ⅰ亚族,此分支被认为介导高亲和性K+。膜结构预测显示该基因编码的蛋白跨膜螺旋区域(TMS)有12个,且在第2和第3 TMS之间有一个较长的胞质质环。洋葱表皮亚细胞定位表明,该基因编码蛋白定位在细胞质膜上;继而对部分转GhHAK1基因拟南芥纯合系进行K+营养试验,发现在低钾(0.05 mmol/L)条件下,野生型拟南芥表现明显的缺钾症状,叶片黄化,植株抽薹明显受抑制,而转基因材料则表现植株叶片生长正常,抽薹未受影响,同时转基因拟南芥叶片中K+含量约为野生型拟南芥的2倍左右,根中K+含量约为1.5倍左右。该结果初步表明GhHAK1基因具有介导植株钾高效吸收的功能,为进一步培育适应土壤钾素匮乏及盐渍化环境下生长的棉花新品种提供重要的基因资源。
Cotton is a typical hi-potassium cash crop, its yield and quality are closely related to the formation of potassium content. The GhHAK1 box of cotton high-affinity potassium transporter gene was cloned and the phylogenetic tree indicated that the GhHAK1 gene belonged to the first subfamily. This branch was thought to mediate the high-affinity K +. Membrane structure prediction showed that the gene encoded 12 transmembrane helix regions (TMS), and had a longer cytoplasmic loop between the 2 and 3 TMS. The epidermal subcellular localization of onion revealed that the gene encodes the protein located on the plasma membrane. Subsequently, K + nutrition test was conducted on the homozygous GhHAK1 transgenic Arabidopsis thaliana and found that under the low potassium (0.05 mmol / L) Arabidopsis showed significant symptoms of potassium deficiency, leaf yellowing, bolting significantly inhibited, while the transgenic material showed normal growth of plant leaves, bolting is not affected, at the same time transgenic Arabidopsis leaves K + content of about wild-type Arabidopsis About 2 times, K + content in the root is about 1.5 times. The results preliminarily indicated that GhHAK1 gene has the function of efficiently absorbing potassium in plants and providing important gene resources for further cultivating new cotton varieties adapted to the conditions of soil potassium deficiency and salinization.