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目的 我们研究了 3个人类结直肠癌细胞系中一组微卫星位点状况和 5 氟尿嘧啶 (5 FU)敏感性的相关性 ,该组位点来自或邻近于肿瘤化疗敏感性可能相关的多个因子。方法 LoVo、SW4 80和SW116细胞系对 5 FU的敏感性经MTT方法测定 ;用免疫组织化学方法检测三细胞系hMSH2、hMLH1表达水平。用PCR SSLP 银染法分别分析了三个细胞系基因组中 10个微卫星位点状况。结果 比较三个细胞系MTT分析获得的IC50 结果发现 ,对 5 FULoVo细胞系较SW4 80和SW116更加敏感 (分别为 :0 .8μmol/L ,2 .2 μmol/L和 1.9μmol/L ,P =0 .0 0 0 )。免疫组织化学检测结果显示hMSH2在SW4 80和SW1116阳性 ,百在LoVo阴性 ;hMLH1在 3个细胞系均阳性。PCR SSLP 银染法结果显示LoVo细胞系在 8/ 10个位点和其余两个细胞系不同 ,电泳条带有不同程度的增加或位移 ,而另外的 2 / 10个位点则具有相同的电泳带型。结论 该组微卫星位点和错配修复状况可以一同作为离体人类结直肠癌细胞系对 5 FU敏感性的指标 ,为了深入阐明它们能否作为临床结直肠癌化疗病人药物筛选和预后评价的有效指标 ,进一步的在体研究和临床资料总结分析很有意义
PURPOSE: We investigated the association of a group of microsatellite loci with 5-fluorouracil (5 FU) sensitivity in three human colorectal cancer cell lines from or adjacent to multiple, potentially related, chemosensitivity chemotherapeutics factor. Methods The sensitivity of LoVo, SW480 and SW116 cells to 5 FU was determined by MTT assay. The expression levels of hMSH2 and hMLH1 in the three cell lines were detected by immunohistochemistry. Ten microsatellite loci in the genome of the three cell lines were analyzed by PCR using SSLP silver staining. Results Comparison of the IC50 results obtained from the MTT assay of the three cell lines revealed that the 5 FULoVo cell line was more sensitive than SW480 and SW116 (0 .8 μmol / L, 2.2 μmol / L and 1.9 μmol / L, respectively, P = 0 .0 0 0). Immunohistochemical results showed that hMSH2 was positive in SW480 and SW1116, negative in LoVo, and hMLH1 was positive in all three cell lines. PCR SSLP silver staining showed that the LoVo cell line was different from the other two cell lines at 8/10 sites with varying degrees of increase or shift in electrophoresis bands while the other 2/10 sites had the same electrophoresis Belt type. Conclusion The microsatellite loci and mismatch repair status in this group can be used as an indicator of sensitivity to 5 FU in vitro in human colorectal cancer cell lines. In order to further elucidate whether they can be used as drug screening and prognostic evaluation for clinical colorectal cancer chemotherapy Effective indicators, further in vivo studies and clinical data summary analysis makes sense