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目的:探讨几种蛋白检测在水泡状胎块(HM)病理诊断与分型中的价值。方法:应用组织芯片及免疫组织化学标记技术,对65例完全性水泡状胎块(CHM)、22例部分性水泡状胎块(PHM)、40例绒毛水肿(HA)共计127例病理组织标本检测p57kip2、CD34、p53、CD117及EGFR的表达。结果:p57kip2在PHM及HA中为阳性表达,在CHM中为阴性表达;CD34在CHM、PHM及HA各组中血管改变情况的评分结果分别为(2.79±0.44)、(2.86±0.36)和(1.23±0.80),在HM组与HA组之间比较差异有统计学意义(P<0.05),在CHM组与PHM组之间比较差异无统计学意义(P>0.05);p53在CHM、PHM及HA各组的评分结果分别为(1.12±0.76)、(0.91±0.53)和(0.65±0.74),在CHM组与HA组之间比较差异有统计学意义(P<0.05),在CHM组与PHM组及PHM组与HA组之间比较,差异均无统计学意义(P>0.05);CD117、EGFR在各组绒毛组织中均为阴性表达。结论:p57kip2、CD34、p53的免疫组织化学检测对于HM的诊断及分型具有重要价值,可作为客观指标应用于临床病理诊断。
Objective: To investigate the value of detecting several proteins in the diagnosis and classification of blister-shaped blocks (HM). Methods: Tissue microarray and immunohistochemical technique were used to detect 127 cases of pathological tissue in 65 cases of complete blister (CHM), 22 cases of partial blister (PHM) and 40 cases of villus edema (HA) The expressions of p57kip2, CD34, p53, CD117 and EGFR were detected. Results: The expression of p57kip2 in PHM and HA was negative, but negative in CHM. The score of CD34 in CHM, PHM and HA groups were (2.79 ± 0.44), (2.86 ± 0.36) and ( 1.23 ± 0.80). The difference between HM group and HA group was statistically significant (P <0.05), but there was no significant difference between CHM group and PHM group (P> 0.05) (1.12 ± 0.76), (0.91 ± 0.53) and (0.65 ± 0.74) in HA group, respectively. There was significant difference between CHM group and HA group (P <0.05). In CHM group There was no significant difference between PHM group and PHM group and HA group (P> 0.05). CD117 and EGFR were negative in each group. Conclusion: The immunohistochemical detection of p57kip2, CD34 and p53 is of great value in the diagnosis and classification of HM, which can be used as an objective indicator in clinicopathological diagnosis.